Caspase-14 Antibody (70A1426) - Azide Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33245

Novus Biologicals, part of Bio-Techne
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NBP2-33245
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 70A1426

Format

Azide Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Caspase-14 Antibodies »

Product Specifications

Immunogen

This antibody was raised against a synthetic peptide corresponding to amino acids 2-18 of mouse Caspase-14.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Caspase-14 Antibody (70A1426) - Azide Free

Western Blot: Caspase-14 Antibody (70A1426)Azide Free [NBP2-33245]

Western Blot: Caspase-14 Antibody (70A1426)Azide Free [NBP2-33245]

Western Blot: Caspase-14 Antibody (70A1426) - Azide Free [NBP2-33245] - Analysis of human breast cancer tissue using Caspase 14 antibody at a concentration of 0.1 ug/ml. Image from the standard format of this antibody.
Flow Cytometry: Caspase-14 Antibody (70A1426) - Azide Free [NBP2-33245]

Flow Cytometry: Caspase-14 Antibody (70A1426) - Azide Free [NBP2-33245]

Flow Cytometry: Caspase-14 Antibody (70A1426) - Azide Free [NBP2-33245] - Intracellular analysis of Caspase-14 in 10^6 Jurkat cells probed with 1:500 dilution of Caspase-14 antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody; red represents Caspase-14 antibody. This antibody was used for this test, and an anti-mouse IgG FITC conjugated secondary antibody. Image from the standard format of this antibody.
Western Blot: Caspase-14 Antibody (70A1426)Azide Free [NBP2-33245]

Western Blot: Caspase-14 Antibody (70A1426)Azide Free [NBP2-33245]

Western Blot: Caspase-14 Antibody (70A1426) - Azide Free [NBP2-33245] - Analysis for Caspase-14 using NB100-56718 at 2 ug/ml dilution against 10 ug of mouse spleen cell lysate. Image from the standard format of this antibody.
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Applications for Caspase-14 Antibody (70A1426) - Azide Free

Application
Recommended Usage

Western Blot

2-4 ug/ml
Application Notes
In mouse spleen, a 30 kDa band is observed.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites with PBS - BSA free

Format

Azide Free

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Caspase-14

Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are constitutively expressed in almost all cell types as inactive proenzymes (zymogens: enzyme precursors which require a biochemical change to become active enzymes) that are processed and activated in response to a variety of pro-apoptotic or inflammatory stimuli. The procaspases (32-56 kDa) contain four domains: an N-terminal prodomain (2-25 kDa), a large subunit (p20: 17-21 kDa), a small subunit (p10: 10-13 kDa) and a short linker region between the large and small subunits. Caspase activation involves proteolytic processing of the proenzyme at specific aspartate residues between the domains. This results in removal of the prodomain as well as the linker region and formation of a heterodimer containing one large and one small subunit (p20-p10). The active caspase is a tetramer composed of two heterodimers (p202-p102). Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. Caspase-14 activation has been implicated in keratinocyte senescence which leads to the cornified cell layer, suggesting a role for caspase-14 in epithelial cell differentiation (Pistritto et al. 2002). Terminal differentiation of keratinocytes has some features of classical apoptosis including DNA fragmentation, nuclear condensation, and induction of cross-linking transglutaminases. Tumor-specific alterations in caspase-14 expression have been found in epithelial malignancies, suggesting that caspase-14 plays a role in epithelial cell transformation. The caspase-14 IMG-5713 antibody recognizes the proform of caspase-14 (approx. 28-32 kDa), and the large (approx. 14-21 kDa) and small (approx. 10-11 kDa) of active/cleaved caspase-14.

Alternate Names

CASP14, Caspase14

Gene Symbol

CASP14

Additional Caspase-14 Products

Product Documents for Caspase-14 Antibody (70A1426) - Azide Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Caspase-14 Antibody (70A1426) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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