Caspase-2 Antibody (18E809.3)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56686

Novus Biologicals, part of Bio-Techne
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NB100-56686
NB100-56686SS
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Kappa Clone # 18E809.3

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Caspase-2 Antibodies »

Product Summary for Caspase-2 Antibody (18E809.3)

Immunogen

A peptide corresponding to amino acids 400-450 of human Caspase-2 was used as immunogen. GenBank no NP_116764.

Reactivity Notes

Predicted to react with Mouse and Rat.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM Kappa

Scientific Data Images for Caspase-2 Antibody (18E809.3)

Western Blot: Caspase-2 Antibody (18E809.3) [NB100-56686]

Western Blot: Caspase-2 Antibody (18E809.3) [NB100-56686]

Western Blot: Caspase-2 Antibody (18E809.3) [NB100-56686] - Analysis for human Caspase-2 using NB100-56686 at 1:1000 on 20 ugs of HL60 whole cell lysate.

Applications for Caspase-2 Antibody (18E809.3)

Application
Recommended Usage

Western Blot

1:500-1:1000
Application Notes
In HL60, a 48 kDa band is observed.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites with PBS and 0.2% Gelatin

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caspase-2

Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-2 has a positive and a negative role in the regulation of the apoptotic process. Overexpression of the full Caspase-2 (Nedd2/Ich1 ) mRNA in some cell types results in apoptosis, whereas overexpression of an alternative Caspase-2 splice variant suppresses apoptosis induced by serum withdrawal. Caspase-2 may be activated in vitro by Caspase-1, Caspase-3 and the neutral serine protease granzyme B which is stored in the specialized lytic granules of cytotoxic lymphocytes.

Alternate Names

CASP2, Caspase2, ICH-1, NEDD2

Entrez Gene IDs

835 (Human)

Gene Symbol

CASP2

UniProt

P42575

Additional Caspase-2 Products

Product Documents for Caspase-2 Antibody (18E809.3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Caspase-2 Antibody (18E809.3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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