Caspase-2 Antibody (3D4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26465
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3D4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human Caspase-2 [UniProt P42575]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Caspase-2 Antibody (3D4)
Immunohistochemistry: Caspase-2 Antibody (3D4) [NBP3-26465] -
Immunohistochemistry: Caspase-2 Antibody (3D4) [NBP3-26465] - Image of Caspase-2 Antibody (3D4) diluted at 1:100 and staining in paraffin-embedded human lung cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: Caspase-2 Antibody (3D4) [NBP3-26465] -
Immunohistochemistry: Caspase-2 Antibody (3D4) [NBP3-26465] - Image of Caspase-2 Antibody (3D4) diluted at 1:100 and staining in paraffin-embedded human lung cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: Caspase-2 Antibody (3D4) [NBP3-26465] -
Western Blot: Caspase-2 Antibody (3D4) [NBP3-26465] - Positive Western Blot detected in: K562 whole cell lysate, 293 whole cell lysate.All lanes: Caspase-2 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 51, 35, 11 kDa
Observed band size: 51 kDa
Applications for Caspase-2 Antibody (3D4)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Caspase-2
Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. They are expressed as latent zymogens and are activated by an autoproteolytic mechanism or by processing by other proteases (frequently other caspases). Human caspases can be subdivided into three functional groups: cytokine activation (caspase-1, -4, -5, and -13), apoptosis initiation (caspase-2, -8, -9, -and -10), and apoptosis execution (caspase-3, -6, and -7).
Caspases are regulated by a variety of stimili, including APAF1, CFLAR/FLIP, NOL3/ARC, and members of the inhibitor of apoptosis (IAP) family such as BIRC1/NAIP, BIRC2/cIAP-1, BIRC3/cIAP-2, BIRC4/XIAP, BIRC5/Survivin, and BIRC7/Livin. IAP activity is modulated by DIABLO/SMAC or PRSS25/HTRA2/Omi. Cell-permeable and irreversible peptide inhibitors are also available for different caspases.
Alternate Names
CASP2, Caspase2, ICH-1, NEDD2
Gene Symbol
CASP2
Additional Caspase-2 Products
Product Documents for Caspase-2 Antibody (3D4)
Product Specific Notices for Caspase-2 Antibody (3D4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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