Caspase-3 Antibody (CPP32 4-1-18) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80614
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # CPP32 4-1-18
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This Caspase-3 Antibody (CPP32 4-1-18) - Azide and BSA Free was developed against full-length recombinant human Caspase 3 [UniProt# P42574].
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
31.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Caspase-3 Antibody (CPP32 4-1-18) - Azide and BSA Free
Western Blot: Caspase-3 Antibody (CPP32 4-1-18)Azide and BSA Free [NBP2-80614]
Western Blot: Caspase-3 Antibody (CPP32 4-1-18) - Azide and BSA Free [NBP2-80614] - Detection of Caspase (19 and 35 kDa) from HEK293 cell extract using Caspase-3 Antibody (CPP32 4-1-18) (NB500-210). Lanes 1 and 2 contain inactive and active Caspase, respectively. Image from the standard format of this antibody.Immunocytochemistry: Caspase-3 Antibody (CPP32 4-1-18) - Azide and BSA Free [NBP2-80614]
Immunocytochemistry: Caspase-3 Antibody (CPP32 4-1-18) - Azide and BSA Free [NBP2-80614] - Caspase-3 was detected in immersion fixed paraffin-embedded sections of human bladder 1:300 dilution of mouse monoclonal Caspase-3 Antibody (CPP32 4-1-18) (NB500-210, Novus Biologicals), for 1 hour at room temperature followed by anti-mouse IgG VisUCyte HImmunohistochemistry-Paraffin: Caspase-3 Antibody (CPP32 4-1-18) - Azide and BSA Free [NBP2-80614]
Immunohistochemistry-Paraffin: Caspase-3 Antibody (CPP32 4-1-18) - Azide and BSA Free [NBP2-80614] - Rat epithelial cells of the tongue base. Antigen retrieval method: Citrate buffer. Image from the standard format of this antibody.Applications for Caspase-3 Antibody (CPP32 4-1-18) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2 ug/ml
Immunohistochemistry
1:200-1:500
Immunohistochemistry-Frozen
reported in scientific literature (PMID 31242448)
Immunohistochemistry-Paraffin
1:200-1:500
Immunoprecipitation
2 ug / mg lysate
Simple Western
1:2000
Western Blot
1:500-1:1000
Application Notes
A band is seen at ~32 kDa for the inactive form of Caspase 3 and ~17-22 kDa for the active form of Caspase 3 in Western Blot. (See protocol for additional information).
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Caspase-3
References
1.Mu, N., Lei, Y., Wang, Y., Wang, Y., Duan, Q., Ma, G., . . . Su, L. (2019). Inhibition of SIRT1/2 upregulates HSPA5 acetylation and induces pro-survival autophagy via ATF4-DDIT4-mTORC1 axis in human lung cancer cells. Apoptosis, 24(9-10), 798-811. doi:10.1007/s10495-019-01559-3
2.Sun, C. M., Enkhjargal, B., Reis, C., Zhou, K. R., Xie, Z. Y., Wu, L. Y., . . . Zhang, J. H. (2019). Osteopontin attenuates early brain injury through regulating autophagy-apoptosis interaction after subarachnoid hemorrhage in rats. CNS Neurosci Ther, 25(10), 1162-1172. doi:10.1111/cns.13199
3.Louneva, N., Cohen, J. W., Han, L. Y., Talbot, K., Wilson, R. S., Bennett, D. A., . . . Arnold, S. E. (2008). Caspase-3 is enriched in postsynaptic densities and increased in Alzheimer's disease. Am J Pathol, 173(5), 1488-1495. doi:10.2353/ajpath.2008.080434
Alternate Names
Apopain, CASP3, Caspase3, CPP32, LICE-1, YAMA
Gene Symbol
CASP3
Additional Caspase-3 Products
Product Documents for Caspase-3 Antibody (CPP32 4-1-18) - Azide and BSA Free
Product Specific Notices for Caspase-3 Antibody (CPP32 4-1-18) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...