Caspase-3 Antibody (S02-7C2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20021

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-20021-100ul
NBP3-20021-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S02-7C2

Concentration

0.3 mg/ml

View all Caspase-3 Antibodies »

Product Summary for Caspase-3 Antibody (S02-7C2)

Immunogen

A synthetic peptide of human Caspase-3 (Uniprot # P42574)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Caspase-3 Antibody (S02-7C2)

Western Blot: Caspase-3 Antibody (S02-7C2) [NBP3-20021]

Western Blot: Caspase-3 Antibody (S02-7C2) [NBP3-20021]

Western Blot: Caspase-3 Antibody (S02-7C2) [NBP3-20021] - Western blot detection of pro Caspase-3 in K562, Hela cell lysates using NBP3-20021 (1:1000 diluted). Predicted band size: 32kDa. Observed band size: 32kDa.
Immunohistochemistry-Paraffin: Caspase-3 Antibody (S02-7C2) [NBP3-20021]

Immunohistochemistry-Paraffin: Caspase-3 Antibody (S02-7C2) [NBP3-20021]

Immunohistochemistry-Paraffin: Caspase-3 Antibody (S02-7C2) [NBP3-20021] - Immunohistochemistry of Caspase-3 in paraffin-embedded Human lung cancer tissue using NBP3-20021 at dilution 1/20

Applications for Caspase-3 Antibody (S02-7C2)

Application
Recommended Usage

Immunohistochemistry

1/20

Western Blot

1/1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caspase-3

CPP32 encodes a member of the cysteine-aspartic acid protease (caspase) family that plays a key role in apoptosis. Caspase 3 (also known as CPP32, Apopain, Yama, and PARP cleavage protease) is the most extensively studied apoptotic protein among caspase family members (1-2). Caspase 3 is synthesized as an inactive proenzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). Alternative splicing of CPP32 results in two transcript variants which encode the same protein. The processed form of Caspase 3 consists of large (theoretical molecular weight 17kD) and small (theoretical molecular weight 12kD) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28/Ser29 and Asp175/Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Variations in levels of Caspase 3 have been reported in cells of short-lived nature and those with a longer life cycle. Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease (3).

References

1.Mu, N., Lei, Y., Wang, Y., Wang, Y., Duan, Q., Ma, G., . . . Su, L. (2019). Inhibition of SIRT1/2 upregulates HSPA5 acetylation and induces pro-survival autophagy via ATF4-DDIT4-mTORC1 axis in human lung cancer cells. Apoptosis, 24(9-10), 798-811. doi:10.1007/s10495-019-01559-3

2.Sun, C. M., Enkhjargal, B., Reis, C., Zhou, K. R., Xie, Z. Y., Wu, L. Y., . . . Zhang, J. H. (2019). Osteopontin attenuates early brain injury through regulating autophagy-apoptosis interaction after subarachnoid hemorrhage in rats. CNS Neurosci Ther, 25(10), 1162-1172. doi:10.1111/cns.13199

3.Louneva, N., Cohen, J. W., Han, L. Y., Talbot, K., Wilson, R. S., Bennett, D. A., . . . Arnold, S. E. (2008). Caspase-3 is enriched in postsynaptic densities and increased in Alzheimer's disease. Am J Pathol, 173(5), 1488-1495. doi:10.2353/ajpath.2008.080434

Alternate Names

Apopain, CASP3, Caspase3, CPP32, LICE-1, YAMA

Gene Symbol

CASP3

Additional Caspase-3 Products

Product Documents for Caspase-3 Antibody (S02-7C2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Caspase-3 Antibody (S02-7C2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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