Caspase 5 Antibody (SR3060)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27741

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-27741-100ul
NBP3-27741-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR3060

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Caspase-5 Antibodies »

Product Summary for Caspase 5 Antibody (SR3060)

Immunogen

A synthesized peptide derived from human Caspase 5 (Uniprot #: P51878)

Specificity

Detects endogenous levels of Caspase 5

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Caspase 5 Antibody (SR3060)

Immunohistochemistry-Paraffin: Caspase 5 Antibody (SR3060) [NBP3-27741] -

Immunohistochemistry-Paraffin: Caspase 5 Antibody (SR3060) [NBP3-27741] - Immunohistochemical analysis of paraffin-embedded human breast cancer.

Western Blot: Caspase 5 Antibody (SR3060) [NBP3-27741] -

Western Blot: Caspase 5 Antibody (SR3060) [NBP3-27741] - Western blot analysis of Caspase 5 expression in HeLa cell lysate.

Applications for Caspase 5 Antibody (SR3060)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caspase-5

Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-5 (ICH-3, ICEREL-III, TY protease) is a member of the caspase-1 subfamily and it is an upstream mediator of apoptosis. Caspase-5 is highly expressed in lung, liver, and skeletal muscle, and similar to Caspase-4, its overexpression can induce cellular apoptosis (1). A tetramer, Caspase-5 separates into two subunits of a 20kda (p20) and 10kda (p10) after activation. In vitro, Caspase-5 can be cleaved by GranB, a major player in Cytotoxic T lymphocytes (CTL) induced apoptosis (2). In vivo, the Caspase-5 expression is elevated by LPS and IFNg. Caspase-5 induced apoptosis can be inhibited by CrmA and Bcl-2. Caspase-5 has also been implicated in cellular inflammatory response and in the microsatelite mutator pathway for cancer (3).

Alternate Names

CASP5, Caspase5, ICE(rel)III, Protease ICH-3, Protease TY

Gene Symbol

CASP5

Additional Caspase-5 Products

Product Documents for Caspase 5 Antibody (SR3060)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Caspase 5 Antibody (SR3060)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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