Caspase-6 Antibody (6D7S2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15771

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15771-100ul
NBP3-15771-20ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6D7S2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Caspase-6 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 45-162 of human Caspase-6 (P55212). GIALIFNHERFFWHLTLPERRGTCADRDNLTRRFSDLGFEVKCFNDLKAEELLLKIHEVSTVSHADADCFVCVFLSHGEGNHIYAYDAKIEIQTLTGLFKGDKCHSLVGKPKIFIIQA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Caspase-6 Antibody (6D7S2)

Western Blot: Caspase-6 Antibody (6D7S2) [NBP3-15771]

Western Blot: Caspase-6 Antibody (6D7S2) [NBP3-15771]

Western Blot: Caspase-6 Antibody (6D7S2) [NBP3-15771] - Western blot analysis of extracts of various cell lines, using Caspase-6 antibody (NBP3-15771) at 1:1000 dilution.Jurkat cells were treated by Staurosporine(1uM) at room temperature for 3 hours . Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: Caspase-6 Antibody (6D7S2) [NBP3-15771]

Immunocytochemistry/ Immunofluorescence: Caspase-6 Antibody (6D7S2) [NBP3-15771]

Immunocytochemistry/Immunofluorescence: Caspase-6 Antibody (6D7S2) [NBP3-15771] - Immunofluorescence analysis of C6 cells using Caspase-6 antibody (NBP3-15771). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Caspase-6 Antibody (6D7S2) [NBP3-15771]

Immunohistochemistry-Paraffin: Caspase-6 Antibody (6D7S2) [NBP3-15771]

Immunohistochemistry-Paraffin: Caspase-6 Antibody (6D7S2) [NBP3-15771] - Immunohistochemistry of paraffin-embedded rat brain using Caspase-6 Rabbit mAb (NBP3-15771) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Applications for Caspase-6 Antibody (6D7S2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Caspase-6

Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-6 is a major apoptotic executioner (1,2). It exists as a pro-domain protein and is cleaved by caspase-3 into two active subunits, an 18 kDa and a 10 kDa subunit (3). Caspase-6 cleaves among many different intracellular proteins, nuclear lamins. Lamins play a significant role in maintaining the integrity of the cell membrane, and their cleavage results in subsequent cellular breakdown and death (4).

Alternate Names

CASP6, Caspase6, Mch2

Gene Symbol

CASP6

Additional Caspase-6 Products

Product Documents for Caspase-6 Antibody (6D7S2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Caspase-6 Antibody (6D7S2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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