Caspase-6 Antibody (6F6J5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33470

Novus Biologicals, part of Bio-Techne
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NBP3-33470-100ul
NBP3-33470-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 6F6J5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Caspase-6 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Caspase-6.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Caspase-6 Antibody (6F6J5)

Caspase-6 Antibody (6F6J5)

Western Blot: Caspase-6 Antibody (6F6J5) [NBP3-33470] -

Western Blot: Caspase-6 Antibody (6F6J5) [NBP3-33470] - Western blot analysis of various lysates, using Caspase-6 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.

Applications for Caspase-6 Antibody (6F6J5)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Caspase-6

Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-6 is a major apoptotic executioner (1,2). It exists as a pro-domain protein and is cleaved by caspase-3 into two active subunits, an 18 kDa and a 10 kDa subunit (3). Caspase-6 cleaves among many different intracellular proteins, nuclear lamins. Lamins play a significant role in maintaining the integrity of the cell membrane, and their cleavage results in subsequent cellular breakdown and death (4).

Alternate Names

CASP6, Caspase6, Mch2

Gene Symbol

CASP6

Additional Caspase-6 Products

Product Documents for Caspase-6 Antibody (6F6J5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Caspase-6 Antibody (6F6J5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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