Caspase-6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56114

Novus Biologicals, part of Bio-Techne
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NB100-56114
NB100-56114SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Cited:

Human, Mouse, Rat, Canine

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Caspase-6 Antibodies »

Product Specifications

Immunogen

Recombinant catalytically active human Caspase-6 protein was used as immunogen.

Reactivity Notes

Cross reacts with Dog, Gerbil, Human, Mouse and Rat. Not yet tested in other species.

Specificity

This polyclonal antisera recognizes the large and small subunits of active/cleaved caspase-6. Whereas the antisera has a strong preference for active/cleaved caspase-6, in some cell or tissue systems or techniques the antisera may also recognize the ~34 kDa proform of caspase-6 as well as intermediate caspase-6 cleavage fragments.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Caspase-6 Antibody

Western Blot: Caspase-6 Antibody [NB100-56114]

Western Blot: Caspase-6 Antibody [NB100-56114]

Western Blot: Caspase-6 Antibody [NB100-56114] - Analysis of Caspase-6 using this antibody. Recombinant catalytically active Caspase-6 was western blotted with Active/Cleaved Caspase-6 antibody. The antibody detected both the large and small subunits of Caspase-6.
Immunohistochemistry-Paraffin: Caspase-6 Antibody [NB100-56114]

Immunohistochemistry-Paraffin: Caspase-6 Antibody [NB100-56114]

Immunohistochemistry-Paraffin: Caspase-6 Antibody [NB100-56114] - Formalin-fixed paraffin-embedded tissue sections of human gliomas stained for Active/Cleaved Caspase-6 expression using Caspase-6 antibody at 1:2000. Gemistocytoma (low grade tumor) at A) low and B) high magnification. Two examples of anaplastic, high grade (III) infiltrating gliomas (C and D). Increased active/cleaved Caspase-6 may be seen in high grade, compared to low grade tumors. Hematoxylin-eosin counterstain.
Immunohistochemistry: Caspase-6 Antibody [NB100-56114]

Immunohistochemistry: Caspase-6 Antibody [NB100-56114]

Immunohistochemistry: Caspase-6 Antibody [NB100-56114] - Formalin-fixed paraffin-embedded tissue section of dog cerebellum stained for Caspase-6 expression at 1:2000. Hematoxylin-eosin counterstain.

Applications for Caspase-6 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000
Application Notes
Immunohistochemistry (frozen): Users should optimize according to model and immunodetection system used (secondary reagents).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

50 ul neat whole antisera

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Caspase-6

Caspases belong to the cysteine-aspartic acid protease (Caspase) family which plays a major role in the transduction of the apoptotic signal and execution of apoptosis in mammalian cells. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein could be processed by caspases 7, 8 and 10, and is thought to function as a downstream. Caspase-3 (CASP3/CPP32) and Caspase-6 (CASP6/Mch2) are the major active caspases in apoptotic cells and are activated in response to distinct apoptosis-inducing stimuli and in all cell, lines analyzed. Both CPP32 and Mch2 are present in apoptotic cells as multiple active species. Mch2 cleaves nuclear mitotic apparatus protein (NuMA) and mediates the shrinkage and fragmentation of nuclei.

Alternate Names

CASP6, Caspase6, Mch2

Entrez Gene IDs

839 (Human); 83584 (Rat)

Gene Symbol

CASP6

Additional Caspase-6 Products

Product Documents for Caspase-6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Caspase-6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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