Caspase-7 Antibody (25B881.1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80615

Novus Biologicals, part of Bio-Techne
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NBP2-80615
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Catalog #
BSA Free
NB100-56529
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 25B881.1

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Caspase-7 Antibodies »

Product Summary for Caspase-7 Antibody (25B881.1) - Azide and BSA Free

Immunogen

This antibody was generated against recombinant protein of active form of human Caspase-7.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Caspase-7 Antibody (25B881.1) - Azide and BSA Free

Western Blot: Caspase-7 Antibody (25B881.1)Azide and BSA Free [NBP2-80615]

Western Blot: Caspase-7 Antibody (25B881.1)Azide and BSA Free [NBP2-80615]

Western Blot: Caspase-7 Antibody (25B881.1) - Azide and BSA Free [NBP2-80615] - Analysis of Caspase-7 in Jurkat cells using Caspase-7 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-7 activation is detected in western blots by the presence of Caspase-7 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-7, depending on the treatment time points. A basal level of endogenous intermediate Caspase-7 can be see in untreated Jurkat cells. Image from the standard format of this antibody.
Immunohistochemistry: Caspase-7 Antibody (25B881.1) - Azide and BSA Free [NBP2-80615]

Immunohistochemistry: Caspase-7 Antibody (25B881.1) - Azide and BSA Free [NBP2-80615]

Immunohistochemistry: Caspase-7 Antibody (25B881.1) - Azide and BSA Free [NBP2-80615] - Human tonsil stained with Caspase-7 antibody at 5 ug/ml. Novus' human tonsil tissue slide was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by co
Western Blot: Caspase-7 Antibody (25B881.1)Azide and BSA Free [NBP2-80615]

Western Blot: Caspase-7 Antibody (25B881.1)Azide and BSA Free [NBP2-80615]

Western Blot: Caspase-7 Antibody (25B881.1) - Azide and BSA Free [NBP2-80615] - Analysis of Caspase-7 in A) human brain, B) mouse brain and C) rat brain lysate using Caspase-7 antibody at 1 ug/ml. Image from the standard format of this antibody.

Applications for Caspase-7 Antibody (25B881.1) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caspase-7

Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-7 is derived from the cleavage of proMCH3-alpha into 2 subunits, p20 and p12 by Caspase-3. Sequence analysis revealed that the MCH3-alpha amino acid sequence had the highest homology to caspase-3 (53% identity) and less than 37% identity with all other family members.

Alternate Names

CASP7, Caspase7, Mch3

Gene Symbol

CASP7

Additional Caspase-7 Products

Product Documents for Caspase-7 Antibody (25B881.1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Caspase-7 Antibody (25B881.1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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