Caspase-7 Antibody (Mch3 1-1-11)

Novus Biologicals, part of Bio-Techne | Catalog # NB500-206

Novus Biologicals, part of Bio-Techne
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NB500-206
NB500-206SS
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Knockdown Validated, Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # Mch3 1-1-11

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Caspase-7 Antibodies »

Product Specifications

Immunogen

Full-length recombinant human Caspase 7 [UniProt# P55210]

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Caspase-7 Antibody (Mch3 1-1-11)

Knockdown Validated: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206]

Knockdown Validated: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206]

Knockdown Validated: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206] - Western blot shows lysates of A549 human lung epithelial parental cell line and Caspase-7 knockout (KO) A549 cell line. PVDF membrane was probed with 1:500 of Mouse Anti-Human Caspase-7 Monoclonal Antibody (Catalog # NB500-106) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Caspase-7 at approximately 40 kDa (as indicated) in the parental A549 cell line, but is not detectable in the knockout A549 cell line. This experiment was conducted under reducing conditions.
Western Blot: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206]

Western Blot: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206]

Western Blot: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206] - HeLa whole cell lysate.
Simple Western: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206]

Simple Western: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206]

Simple Western: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206] - Simple Western lane view shows a specific band for Caspase 7 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing A246 conditions using the 12-230 kDa separation system.

Applications for Caspase-7 Antibody (Mch3 1-1-11)

Application
Recommended Usage

Simple Western

1:500

Western Blot

1:500-1:1000
Application Notes
This Caspase 7 (Mch3 1-1-11) antibody is useful for Western blot, where a band can be seen at approx. 35 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caspase-7

Caspases are evolutionary conserved family of aspartate-specific cystein proteases which are essential part of apoptotic machinery and typically signal in a two-step cascade: 'initiator caspases' with large prodomains (such as caspase-1, -8 and -9) are first recruited into large protein complexes in which they undergo proximity-induced autoactivation and "executioner caspases" such as caspase-3 and -6, caspase-7 which are activated by initiator caspases via freeing of executioner caspases of their short inhibitory prodomain, allowing them to cleave a large set of cellular substrates. Localized in cytoplasm, caspase 7 is a heterotetramer that is consists of two anti-parallel arranged heterodimers, each one formed by a 20 kD (p20) and a 11 kD (p11) subunit, and interacts with BIRC6/bruce. Caspase 7 cleaves/activates SREBPs and poly(ADP-ribose) polymerase (PARP). Its overexpression promotes apoptosis, and it also implicates in inflammation signaling. Therapeutic intervention with caspase-7 has been shown to prove beneficial in conditions where excessive cell death and/or inflammation contribute to diseases e.g. Alzheimer's disease, Huntington's disease, sepsis, rheumatoid arthritis, cancer and NIDDM.

Alternate Names

CASP7, Caspase7, Mch3

Entrez Gene IDs

840 (Human); 12369 (Mouse); 64026 (Rat)

Gene Symbol

CASP7

UniProt

P55210 (Mouse)

Additional Caspase-7 Products

Product Documents for Caspase-7 Antibody (Mch3 1-1-11)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Caspase-7 Antibody (Mch3 1-1-11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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