Caspase-8 Antibody (FLICE 4-1-20)

Novus Biologicals, part of Bio-Techne | Catalog # NB500-208

Novus Biologicals, part of Bio-Techne
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NB500-208
NB500-208SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # FLICE 4-1-20

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Caspase-8 Antibodies »

Product Specifications

Immunogen

Full-length recombinant human Caspase 8 [UniProt# Q14790]

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29069785).

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Caspase-8 Antibody (FLICE 4-1-20)

Western Blot: Caspase-8 Antibody (FLICE 4-1-20) [NB500-208]

Western Blot: Caspase-8 Antibody (FLICE 4-1-20) [NB500-208]

Western Blot: Caspase-8 Antibody (FLICE 4-1-20) [NB500-208] - CaSki cells were mock-infected or exposed to each of the indicated viruses (MOI ~ 1 pfu/cell) or 10 uM staurosporine and 4 h pi, cellular lysates were prepared and analyzed by immunoblotting for Caspase-8. Image collected and cropped by CiteAb from the following publication (https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006766) licensed under a CC-BY license.
Western Blot: Caspase-8 Antibody (FLICE 4-1-20) [NB500-208]

Western Blot: Caspase-8 Antibody (FLICE 4-1-20) [NB500-208]

Western Blot: Caspase 8 Antibody (FLICE 4-1-20) [NB500-208] - Detection of Caspase 8 (59 kDa) from HEK293 cell extract using NB500-208 (1:500). Lanes 1 and 2 contain inactive and active Caspase 8, respectively.

Applications for Caspase-8 Antibody (FLICE 4-1-20)

Application
Recommended Usage

Western Blot

1:1000
Application Notes
This Caspase 8 (FLICE 4-1-20) antibody is useful for Western Blot where a band is seen at 55 kDa representing the inactive form of Caspase 8 (see protocol for additional information). We also have reports that the antibody detects the active (pro) form of Caspase 8.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Caspase-8

Caspases are evolutionary conserved family of aspartate-specific cystein proteases which are essential part of apoptotic machinery and typically signal in a two-step cascade: 'initiator caspases' with large prodomains (such as caspase-1, -8 and -9) are first recruited into large protein complexes in which they undergo proximity-induced autoactivation and "executioner caspases" such as caspase-3 and -6, caspase-7 which are activated by initiator caspases via freeing of executioner caspases of their short inhibitory prodomain, allowing them to cleave a large set of cellular substrates. Localized in cytoplasm, caspase 7 is a heterotetramer that is consists of two anti-parallel arranged heterodimers, each one formed by a 20 kD (p20) and a 11 kD (p11) subunit, and interacts with BIRC6/bruce. Caspase 7 cleaves/activates SREBPs and poly(ADP-ribose) polymerase (PARP). Its overexpression promotes apoptosis, and it also implicates in inflammation signaling. Therapeutic intervention with caspase-7 has been shown to prove beneficial in conditions where excessive cell death and/or inflammation contribute to diseases e.g. Alzheimer's disease, Huntington's disease, sepsis, rheumatoid arthritis, cancer and NIDDM.

Alternate Names

CASP8, Caspase8, Mch5

Entrez Gene IDs

841 (Human)

Gene Symbol

CASP8

UniProt

Q14790

Additional Caspase-8 Products

Product Documents for Caspase-8 Antibody (FLICE 4-1-20)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Caspase-8 Antibody (FLICE 4-1-20)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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