Caspase-8 Antibody - (Pro and Active)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05123
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
IF/IHC, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Synthetic peptide corresponding to the C-terminus of human Caspase-8 (PQPTFTLRKKLVFPSD). This sequence is common to various Caspase-8 forms including: 1. Caspase 8a (aa 481-496 GenBank no. NP_001219.2) 2. Caspase 8b (aa 464-479, GenBank no. NP_203519.1) 3. Caspase 8c (aa 449-464, GenBank no. NP_203520.1) 4. Caspase 8L (aa 523-538, GenBank no. gbAAL87628.1) 5. Truncated caspase 8 (as 251-266, GenBank no. gi3928274)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Caspase-8 Antibody - (Pro and Active)
Immunohistochemistry-Paraffin: Caspase-8 Antibody - (Pro and Active) [NBP1-05123]
Immunohistochemistry-Paraffin: Caspase-8 Antibody - (Pro and Active) [NBP1-05123] - Formalin-fixed, paraffin-embedded sections from a brain tumor tissue array stained for Caspase-8 expression using NBP1-05123 at 1:2000. A. Anaplastic glioma cores from two different patients, positive (left) and negative (right) staining for caspase-8. B. Higher magnification from the caspase-8 positive (A, left) core.Western Blot: Caspase-8 Antibody(Pro and Active) [NBP1-05123]
Western Blot: Caspase-8 Antibody - (Pro and Active) [NBP1-05123] - AML12 whole cell lysate. Good specific band is observed. Primary antibody at 1: 2000, secondary antibody at 1: 20000. Skimmed milk as blocking buffer. Western blot image submitted by a verified customer review.Applications for Caspase-8 Antibody - (Pro and Active)
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:1000-1:5000
Immunoprecipitation
1:50-1:200
Western Blot
1:1000-1:2000
Reviewed Applications
Read 1 review rated 5 using NBP1-05123 in the following applications:
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Whole antisera
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Caspase-8
Alternate Names
CASP8, Caspase8, Mch5
Gene Symbol
CASP8
UniProt
Additional Caspase-8 Products
Product Documents for Caspase-8 Antibody - (Pro and Active)
Product Specific Notices for Caspase-8 Antibody - (Pro and Active)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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