Caspase-8 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76610

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Caspase-8 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Caspase-8 isoform A. The immunogen is located within the last 50 amino acids of Caspase-8. Amino Acid Squence: NYEVSNKDDKKNMGKQ

Specificity

Depending on cell lines or tissues used, either full-length or other cleavage products may be observed.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Caspase-8 Antibody - BSA Free

Western Blot: Caspase-8 AntibodyBSA Free [NBP1-76610]

Western Blot: Caspase-8 AntibodyBSA Free [NBP1-76610]

Western Blot: Caspase-8 Antibody [NBP1-76610] - Analysis of Caspase-8 in (A) human spleen, (B) mouse spleen, and (C) rat spleen tissue lysate with Caspase-8 antibody at 0.5 ug/ml.
Immunohistochemistry: Caspase-8 Antibody - BSA Free [NBP1-76610]

Immunohistochemistry: Caspase-8 Antibody - BSA Free [NBP1-76610]

Immunohistochemistry: Caspase-8 Antibody [NBP1-76610] - Staining of human spleen tissue with antibody at 5 ug/mL.
Western Blot: Caspase-8 AntibodyBSA Free [NBP1-76610]

Western Blot: Caspase-8 AntibodyBSA Free [NBP1-76610]

Western Blot: Caspase-8 Antibody [NBP1-76610] - Analysis in Jurkat cell lysate with antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
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Applications for Caspase-8 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

2-20 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

0.5-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caspase-8

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-8 is an initiator caspase that was identified as a member of the Fas/APO-1 death-inducing signaling complex. The adaptor molecule FADD couples procaspase-8 to the Fas receptor death domain; subsequent oligomerization promotes procaspase-8 autoactivation. FLIP, a catalytically inactive caspase-8-like molecule inhibits these interactions and thus can inhibit apoptosis.

Alternate Names

CASP8, Caspase8, Mch5

Gene Symbol

CASP8

UniProt

NP_001219 (Human)

Additional Caspase-8 Products

Product Documents for Caspase-8 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Caspase-8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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