Caspase-8 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88185
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GIIYGTDGQEAPIYELTSQFTGLKCPSLAGKPKVFFIQACQGDNYQKGIPVETDSEEQPYLEMDLSSPQTRYIPDEADFLLGMATVNNCVSYRNPAEGTWYIQSLCQSLRERCPRGDDILTILTEVNYEVSNKDDKKNMGKQMPQP
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Caspase-8 Antibody
![Western Blot: Caspase-8 Antibody [NBP1-88185]](https://resources.bio-techne.com/images/products/Caspase-8-Antibody-Western-Blot-NBP1-88185-img0006.jpg "Western Blot: Caspase-8 Antibody [NBP1-88185]")
Western Blot: Caspase-8 Antibody [NBP1-88185]
Western Blot: Caspase-8 Antibody [NBP1-88185] - Analysis in human cell line RPMI-8226.![Immunocytochemistry/ Immunofluorescence: Caspase-8 Antibody [NBP1-88185]](https://resources.bio-techne.com/images/products/Caspase-8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88185-img0007.jpg "Immunocytochemistry/ Immunofluorescence: Caspase-8 Antibody [NBP1-88185]")
Immunocytochemistry/ Immunofluorescence: Caspase-8 Antibody [NBP1-88185]
Immunocytochemistry/Immunofluorescence: Caspase-8 Antibody [NBP1-88185] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.![Immunohistochemistry-Paraffin: Caspase-8 Antibody [NBP1-88185]](https://resources.bio-techne.com/images/products/Caspase-8-Antibody-Immunohistochemistry-Paraffin-NBP1-88185-img0008.jpg "Immunohistochemistry-Paraffin: Caspase-8 Antibody [NBP1-88185]")
Immunohistochemistry-Paraffin: Caspase-8 Antibody [NBP1-88185]
Immunohistochemistry-Paraffin: Caspase-8 Antibody [NBP1-88185] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center and non-germinal center cells.Applications for Caspase-8 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Caspase-8
Caspase 8 is expressed as an inactive proenzyme which then undergos proteolytic processing by upstream caspases, to form an active (cleaved) caspase 8 form.
Caspase 8 antibodies are important tools for apoptosis studies and research on the caspase family mediated apoptosis signaling pathway.
Alternate Names
CASP8, Caspase8, Mch5
Gene Symbol
CASP8
Additional Caspase-8 Products
Product Documents for Caspase-8 Antibody
Product Specific Notices for Caspase-8 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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