CASZ1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77942
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for CASZ1 Antibody
Immunogen
This affinity purified CASZ1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to region near the amino terminal end of human CASZ1 protein. (Uniprot: B3KRV8)
Reactivity Notes
A BLAST analysis was used to suggest cross reactivity with CASZ1 from human, mouse, chimpanzee, bovine, and rat based on 100% homology with the immunizing sequence. Expect cross reactivity with multiple isoforms of this protein. Cross reactivity with CASZ1 from other sources has not been determined.
Specificity
Expect cross reactivity with multiple isoforms of this protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This affinity-purified antibody is directed against human CASZ1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for CASZ1 Antibody
![Western Blot: CASZ1 Antibody [NBP1-77942]](https://resources.bio-techne.com/images/products/MAGP-2-MFAP5-Antibody-Western-Blot-NBP1-77942-img0002.jpg "Western Blot: CASZ1 Antibody [NBP1-77942]")
Western Blot: CASZ1 Antibody [NBP1-77942]
Western Blot: MAGP-2/MFAP5 Antibody [NBP1-77942] - Shows detection of a band ~125 kDa in size corresponding to hCas5 (arrowhead). Lane 1 contains a lysate from AS cells transfected with empty vector. Lane 2 contains lysate from AS cells transfected with hCas5 (predicted MW 125 kDa). Lane 3 contains lysate from BE2 cells that have been shown to contain a high level of hCas mRNA (as tested by Northern blot). Lane 4 contains lysate from human muscle tissue that also shows high levels of hCas mRNA. Specific band reactivity is effectively competed when the antibody is pre-incubated with the immunizing peptide (data not shown). Personal Communication. Carol Thiele, NCI, Bethesda, MD.
CASZ1 Antibody
Western blot using affinity purified anti-hCas5 antibody. Lane 1: AS cell lysate transfected with empty vector. Lane 2: AS cell lysate transfected with hCas5 (predicted MW 125kDa). Lane 3: BE2 cell lysate that have been shown to contain a high level of hCas mRNA (as tested by Northern blot). Lane 4: human muscle tissue lysate that shows high levels of hCas mRNA. Shows detection of a band ~125kDa in size corresponding to hCas5 in lane 2 (arrowhead). Specific band reactivity is effectively competed when the antibody is pre-incubated with the immunizing peptide (data not shown). Personal Communication. Carol Thiele, NCI, Bethesda, MD.Applications for CASZ1 Antibody
Application
Recommended Usage
ELISA
1:10000-1:50000
Western Blot
1:500-1:2000
Application Notes
This affinity-purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot, a band approximately 125 kDa in size corresponding to hCas5 protein is expected in the appropriate cell lysate or extract.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: CASZ1
developmentally regulated transcription factor found in neural and muscle cells. hCas5 is a Zn finger transcription factor that is predominantly found in the nucleus. This antibody is designed to facilitate the functional study of this protein and its role in developmental regulation and expression in human tumor tissues.
Alternate Names
castor, castor homolog 1, zinc finger, castor homolog 1, zinc finger (Drosophila), castor zinc finger 1, Castor-related protein, CST, dJ734G22.1, FLJ20321, Putative survival-related protein, SRG, SRGcst, survival related, survival-related, Zinc finger protein 693, zinc finger protein castor homolog 1, ZNF693, ZNF693FLJ12223
Gene Symbol
CASZ1
UniProt
Additional CASZ1 Products
Product Documents for CASZ1 Antibody
Product Specific Notices for CASZ1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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