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CAT1 Antibody (2B9)

Novus Biologicals, part of Bio-Techne | Catalog # H00006541-M02

Novus Biologicals, part of Bio-Techne
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H00006541-M02

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2B9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

SLC7A1 (NP_003036, 431 a.a. ~ 492 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RYQPEQPNLVYQMASTSDELDPADQNELASTNDSQLGFLPEAEMFSLKTILSPKNMEPSKIS

Reactivity Notes

Mouse data from journals.plos.org/plosone/article?id=10.1371/journal.pone.0170456.

Specificity

Reacts with solute carrier family 7 (cationic amino acid transporter, y+ system), member 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CAT1 Antibody (2B9)

Western Blot: CAT1 Antibody (2B9) [H00006541-M02]

Western Blot: CAT1 Antibody (2B9) [H00006541-M02]

Western Blot: CAT1 Antibody (2B9) [H00006541-M02] - Since miRNA214 is upregulated and miRNA 378 is downregulated following 12 days of TAC, western immunoblotting was carried out for nodal molecule (NF kappa B) and other network molecules (HDGF, BCL2 and SLC7A1). GAPDH was used a loading control, *p<0.05 vs. Sham, (n = 8). Image collected and cropped by CiteAb from the following publication (journals.plos.org/plosone/article?id=10.1371/journal.pone.0170456), licensed under a CC-BY license.
Western Blot: CAT1 Antibody (2B9) [H00006541-M02]

Western Blot: CAT1 Antibody (2B9) [H00006541-M02]

Western Blot: CAT1 Antibody (2B9) [H00006541-M02] - Analysis of SLC7A1 expression in transfected 293T cell line by SLC7A1 monoclonal antibody (M02), clone 2B9.Lane 1: SLC7A1 transfected lysate (Predicted MW: 67.6 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: CAT1 Antibody (2B9) [H00006541-M02] - Detection limit for recombinant GST tagged SLC7A1 is 0.03 ng/ml as a capture antibody.

Applications for CAT1 Antibody (2B9)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in WB and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CAT1/SLC7A1

High-affinity, low capacity permease involved in the transport of the cationic amino acids (arginine, lysine and ornithine) in non-hepatic tissues. May also function as an ecotropic retroviral leukemia receptor

Long Name

Cationic 1/Solute Carrier Family 7 Member 1

Alternate Names

ATRC1, HCAT1, REC1L, SLC7A1

Entrez Gene IDs

6541 (Human)

Gene Symbol

SLC7A1

UniProt

Additional CAT1/SLC7A1 Products

Product Documents for CAT1 Antibody (2B9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CAT1 Antibody (2B9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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