Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all CAT1/SLC7A1 Antibodies »

Product Summary for CAT1 Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 600 to 650 of human High Affinity Cationic Amino Acid Transporter 1 using the numbering given in entry NP_003036.1 (GeneID 6541).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CAT1 Antibody

Western Blot: CAT1 Antibody [NBP2-32271]

Western Blot: CAT1 Antibody [NBP2-32271]

Western Blot: CAT1 Antibody [NBP2-32271] - Samples: Whole cell lysate (50 ug) prepared using RIPA buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CAT-1 antibody NBP2-32271 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: CAT1 Antibody [NBP2-32271]

Immunoprecipitation: CAT1 Antibody [NBP2-32271]

Immunoprecipitation: CAT1 Antibody [NBP2-32271] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-CAT-1 antibody NBP2-32271 used for IP at 6 ug per reaction. CAT-1 was also immunoprecipitated by rabbit anti-CAT-1 antibody BL16464. For blotting immunoprecipitated CAT-1, NBP2-32271 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for CAT1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CAT1/SLC7A1

High-affinity, low capacity permease involved in the transport of the cationic amino acids (arginine, lysine and ornithine) in non-hepatic tissues. May also function as an ecotropic retroviral leukemia receptor

Long Name

Cationic 1/Solute Carrier Family 7 Member 1

Alternate Names

ATRC1, HCAT1, REC1L, SLC7A1

Gene Symbol

SLC7A1

UniProt

NP_003036.1 (Human)

Additional CAT1/SLC7A1 Products

Product Documents for CAT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CAT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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