Key Product Details

Species Reactivity

H. pylori

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # HPYL/7170

Format

Azide and BSA Free

Concentration

1 mg/ml

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Product Specifications

Immunogen

Recombinant Helicobacter pylori Catalase protein fragment (around aa323-445) (Exact sequence is proprietary)

Reactivity Notes

Helicobacter pylori

Localization

Cell surface, Cytoplasm

Specificity

The spiral shaped bacterium Helicobacter pyloriis strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy.H. pyloriis known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13821).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Applications for Catalase Antibody (HPYL/7170) - (H. pylori) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Catalase

Human catalase is an heme-containing peroxisomal enzyme that breaks down hydrogen peroxide to water and oxygen; it is implicated in ethanol metabolism, inflammation, apoptosis, aging and cancer. The 1.5A resolution human enzyme structure, both with and without bound NADPH, establishes the conserved features of mammalian catalase fold and assembly, implicates Tyr370 as the tyrosine radical, suggests the structural basis for redox-sensitive binding of cognate mRNA via the catalase NADPH binding site, and identifies an unexpectedly substantial number of water-mediated domain contacts (1). Because catalase has a low affinity for H2O2, it has been suggested that glutathione peroxidase clears most H2O2 within the erthrocyte and that catalase is of little import. It has been hypothesized that erythrocyte catalase might function to protect heterologous somatic cells against challenge by high levels of exogenous H2O2 (e.g., in areas of inflamation) (2).

Alternate Names

Cas1, CAT, Cs-1

Gene Symbol

CAT

Additional Catalase Products

Product Documents for Catalase Antibody (HPYL/7170) - (H. pylori) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Catalase Antibody (HPYL/7170) - (H. pylori) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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