Catenin alpha 1 Antibody (1G5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54533

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1G5

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Catenin alpha 1 Antibodies »

Product Specifications

Immunogen

Human alpha catenin fusion protein (Uniprot: P35221)

Localization

Cell Surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

102 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53289).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Catenin alpha 1 Antibody (1G5) - Azide and BSA Free

Western Blot: Catenin alpha 1 Antibody (1G5)Azide and BSA Free [NBP2-54533]

Western Blot: Catenin alpha 1 Antibody (1G5)Azide and BSA Free [NBP2-54533]

Western Blot: Catenin alpha 1 Antibody (1G5) - Azide and BSA Free [NBP2-54533] - Western Blot of A431 and MCF-7 cell lysate using Catenin alpha 1 Antibody (1G5).

Applications for Catenin alpha 1 Antibody (1G5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells

Immunocytochemistry/ Immunofluorescence

1 - 2 ug/ml

Western Blot

0.5 - 1.0 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Catenin alpha 1

Cadherin and catenin compose cell adhesion complex and are indispensable for tight cell-cell adhesion. Dysfunction of this adhesion complex causes dissociation of cancer cells from primary tumor nodules, thus possibly contributing to cancer invasion and metastasis (1). At least three proteins (alpha, beta, and gamma catenin) comprise the cytoplasmic domain of the cadherin cell-cell adhesion complex. Data, with the reported structure of other catenin genes, suggest that vinculin and alpha-catenin generate a superfamily of proteins mediating membrane-cytoskeletal associations (2). Presenilin-1 (PS1) overexpression in human kidney cells enhances cell-cell adhesion and data show that PS1 incorporates into the cadherin/catenin adhesion system and regulates cell-cell adhesion. PS1 concentrates at intercellular contacts in epithelial tissue; in brain, it forms complexes with both E- and N-cadherin and concentrates at synaptic adhesions (3).

Alternate Names

Alpha E-catenin, alpha-catenin, alpha-E-catenin, alphaE-catenin, Cadherin-associated protein, CAP102, catenin (cadherin-associated protein), alpha 1 (102kD), catenin (cadherin-associated protein), alpha 1, 102kDa, catenin alpha-1, FLJ36832, FLJ52416, Renal carcinoma antigen NY-REN-13

Gene Symbol

CTNNA1

Additional Catenin alpha 1 Products

Product Documents for Catenin alpha 1 Antibody (1G5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Catenin alpha 1 Antibody (1G5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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