Catenin alpha 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB120-11346

Novus Biologicals, part of Bio-Techne
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NB120-11346
NB120-11346-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Microarray, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Catenin alpha 1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide: EKQDETQTKIKRASQ conjugated to KLH, corresponding to amino acids 873-887 of Human Catenin alpha 1 Antibody.

Reactivity Notes

This sequence is identical in mouse and Xenopus alpha-E-catenin. It is not found in alpha-N-catenin, beta-catenin, and gamma-catenin.

Localization

Cell Membrane and Cytoplasmic

Specificity

Catenin alpha 1 recognizes alpha-E-catenin (102 kDa).Staining of the Catenin alpha 1 band in immunoblotting is specifically inhibited with the Catenin alpha 1 immunizing peptide (human, amino acids 873-887).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Catenin alpha 1 Antibody

Western Blot: Catenin alpha 1 Antibody [NB120-11346]

Western Blot: Catenin alpha 1 Antibody [NB120-11346]

Western Blot: Catenin alpha 1 Antibody [NB120-11346] - Rat embryonic brain (S1) extract was separated on SDS-PAGE and probed with IGG FRACTION AB TO alpha-E-CATENIN (RB). The antibody was developed with HRP Anti-Rabbit IgG and an ECL substrate. Antibody dilution 1:2000.
Immunocytochemistry/ Immunofluorescence: Catenin alpha 1 Antibody [NB120-11346]

Immunocytochemistry/ Immunofluorescence: Catenin alpha 1 Antibody [NB120-11346]

Immunocytochemistry/Immunofluorescence: Catenin alpha 1 Antibody [NB120-11346] - MDCK Madin Darby Canine Kidney cells were fixed and permeabilized with methanol. Fixed cells were stained with 1:100 Anti-alpha-E-Catenin antibody produced in Rabbit The antibody was developed using 1:500 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat.

Applications for Catenin alpha 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
WB: Detects a band of approximately 102 kDa.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

10mM PBS (pH 7.4)

Preservative

0.9% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Catenin alpha 1

Cadherin and catenin compose cell adhesion complex and are indispensable for tight cell-cell adhesion. Dysfunction of this adhesion complex causes dissociation of cancer cells from primary tumor nodules, thus possibly contributing to cancer invasion and metastasis (1). At least three proteins (alpha, beta, and gamma catenin) comprise the cytoplasmic domain of the cadherin cell-cell adhesion complex. Data, with the reported structure of other catenin genes, suggest that vinculin and alpha-catenin generate a superfamily of proteins mediating membrane-cytoskeletal associations (2). Presenilin-1 (PS1) overexpression in human kidney cells enhances cell-cell adhesion and data show that PS1 incorporates into the cadherin/catenin adhesion system and regulates cell-cell adhesion. PS1 concentrates at intercellular contacts in epithelial tissue; in brain, it forms complexes with both E- and N-cadherin and concentrates at synaptic adhesions (3).

Alternate Names

Alpha E-catenin, alpha-catenin, alpha-E-catenin, alphaE-catenin, Cadherin-associated protein, CAP102, catenin (cadherin-associated protein), alpha 1 (102kD), catenin (cadherin-associated protein), alpha 1, 102kDa, catenin alpha-1, FLJ36832, FLJ52416, Renal carcinoma antigen NY-REN-13

Entrez Gene IDs

1495 (Human)

Gene Symbol

CTNNA1

Additional Catenin alpha 1 Products

Product Documents for Catenin alpha 1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Catenin alpha 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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