Catenin alpha 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33456

Novus Biologicals, part of Bio-Techne
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NBP2-33456
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Catenin alpha 1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LADMADVYKLLVQLKVVEDGILKLRNAGNEQDLGIQYKALKPEVDKLNIMAAK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Catenin alpha 1 Antibody

Western Blot: Catenin alpha 1 Antibody [NBP2-33456]

Western Blot: Catenin alpha 1 Antibody [NBP2-33456]

Western Blot: Catenin alpha 1 Antibody [NBP2-33456] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: Catenin alpha 1 Antibody [NBP2-33456]

Immunocytochemistry/ Immunofluorescence: Catenin alpha 1 Antibody [NBP2-33456]

Immunocytochemistry/Immunofluorescence: Catenin alpha 1 Antibody [NBP2-33456] - Immunofluorescent staining of human cell line CACO-2 shows localization to plasma membrane & cell junctions.
Immunohistochemistry: Catenin alpha 1 Antibody [NBP2-33456]

Immunohistochemistry: Catenin alpha 1 Antibody [NBP2-33456]

Immunohistochemistry: Catenin alpha 1 Antibody [NBP2-33456] - Staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.

Applications for Catenin alpha 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Catenin alpha 1

Cadherin and catenin compose cell adhesion complex and are indispensable for tight cell-cell adhesion. Dysfunction of this adhesion complex causes dissociation of cancer cells from primary tumor nodules, thus possibly contributing to cancer invasion and metastasis (1). At least three proteins (alpha, beta, and gamma catenin) comprise the cytoplasmic domain of the cadherin cell-cell adhesion complex. Data, with the reported structure of other catenin genes, suggest that vinculin and alpha-catenin generate a superfamily of proteins mediating membrane-cytoskeletal associations (2). Presenilin-1 (PS1) overexpression in human kidney cells enhances cell-cell adhesion and data show that PS1 incorporates into the cadherin/catenin adhesion system and regulates cell-cell adhesion. PS1 concentrates at intercellular contacts in epithelial tissue; in brain, it forms complexes with both E- and N-cadherin and concentrates at synaptic adhesions (3).

Alternate Names

Alpha E-catenin, alpha-catenin, alpha-E-catenin, alphaE-catenin, Cadherin-associated protein, CAP102, catenin (cadherin-associated protein), alpha 1 (102kD), catenin (cadherin-associated protein), alpha 1, 102kDa, catenin alpha-1, FLJ36832, FLJ52416, Renal carcinoma antigen NY-REN-13

Gene Symbol

CTNNA1

UniProt

P35221 (Human)

Additional Catenin alpha 1 Products

Product Documents for Catenin alpha 1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Catenin alpha 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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