Cathepsin L Antibody (221)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89528

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 221

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

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Product Summary for Cathepsin L Antibody (221)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Cathepsin L (Accession#: NP_001903.1; Met1-Val333).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Cathepsin L Antibody (221)

Western Blot: Cathepsin L Antibody (221) [NBP2-89528]

Western Blot: Cathepsin L Antibody (221) [NBP2-89528]

Western Blot: Cathepsin L Antibody (221) [NBP2-89528] - Lane A: HepG2 Whole Cell Lysate Lane B: A549 Whole Cell LysateLane C: HCT116 Whole Cell Lysate Lane D: U87MG Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:36 kDaObserved band size:25 kDa
Cathepsin L Antibody (221) Western Blot: Cathepsin L Antibody (221) [NBP2-89528]

Western Blot: Cathepsin L Antibody (221) [NBP2-89528]

NBP2-89528 used at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lane B: Cathepsin L knockout A549 Whole Cell Lysate. Lysates loaded at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)[HRP] used at at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 25 kDa.

Applications for Cathepsin L Antibody (221)

Application
Recommended Usage

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cathepsin L

Cathepsins are lysosomal proteases that play an important role in the intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumour invasion and metastasis. Normally located in lysosomes of almost all mammalian cells, they can be secreted from the cell under certain conditions. Cathepsin L is responsible for most of the intralysosomal breakdown of normal cells. Cathepsin L is secreted by numerous transformed cells in its inactive proform, and mRNA expression level seems to correlate with their metastatic potential.

Alternate Names

CTSL, CTSL1

Gene Symbol

CTSL

Additional Cathepsin L Products

Product Documents for Cathepsin L Antibody (221)

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Certificate of Analysis

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Product Specific Notices for Cathepsin L Antibody (221)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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