Cathepsin L Antibody (PSH03-92)

Western Blot: Cathepsin L Antibody (PSH03-92) [NBP3-32112]

Western Blot: Cathepsin L Antibody (PSH03-92) [NBP3-32112] - Western blot analysis of Cathepsin L on different lysates with Rabbit anti-Cathepsin L antibody (NBP3-32112) at 1/5,000 dilution.

Lane 1: A549 cell lysate (15 ug/Lane)
Lane 2: HepG2 cell lysate (15 ug/Lane)
Lane 3: HEK-293 cell lysate (15 ug/Lane)
Lane 4: MDA-MB-231 cell lysate (15 ug/Lane)
Lane 5: Human kidney tissue lysate (30 ug/Lane)
Lane 6: Human liver tissue lysate (30 ug/Lane)
Lane 7: NIH/3T3 cell lysate (15 ug/Lane)
Lane 8: PC-12 cell lysate (15 ug/Lane)
Lane 9: Mouse kidney tissue lysate (30 ug/Lane)
Lane 10: Rat kidney tissue lysate (30 ug/Lane)
Lane 11: Mouse liver tissue lysate (30 ug/Lane)
Lane 12: Rat liver tissue lysate (30 ug/Lane)
Lane 13: COS-1 cell lysate (15 ug/Lane)

Predicted band size: 38 kDa
Observed band size: 20-45 kDa

Exposure time: 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32112) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Cathepsin L Antibody (PSH03-92) [NBP3-32112]

Immunohistochemistry: Cathepsin L Antibody (PSH03-92) [NBP3-32112] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Cathepsin L antibody (NBP3-32112) at 1/10,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32112) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.