Cav1.3 Antibody (S48)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12893
![Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893]](https://resources.bio-techne.com/images/products/Cav1-3-Antibody-S48-Immunohistochemistry-NBP2-12893-img0005.jpg "Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893]")
![Immunocytochemistry/ Immunofluorescence: Cav1.3 Antibody (S48) [NBP2-12893]](https://resources.bio-techne.com/images/products/Cav1-3-Antibody-S48-Immunocytochemistry-Immunofluorescence-NBP2-12893-img0007.jpg "Immunocytochemistry/ Immunofluorescence: Cav1.3 Antibody (S48) [NBP2-12893]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Microarray, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # S48
Concentration
1 mg/ml
Product Specifications
Immunogen
Fusion protein amino acids 859-875 of rat Cav1.3
Localization
Membrane, Cell Membrane
Specificity
Detects approx 250 kDa. No cross-reactivity against Cav1.2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for Cav1.3 Antibody (S48)
Immunocytochemistry/ Immunofluorescence: Cav1.3 Antibody (S48) [NBP2-12893]
Immunocytochemistry/Immunofluorescence: Cav1.3 Antibody (S48) [NBP2-12893] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.3Monoclonal Antibody, Clone S48 (NBP2-12893). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav1.3Monoclonal Antibody (NBP2-12893) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav1.3Antibody (D) Composite.![Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893]](https://resources.bio-techne.com/images/products/Cav1-3-Antibody-S48-Immunohistochemistry-NBP2-12893-img0006.jpg "Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893]")
Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893]
Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893] - Immunohistochemistry analysis using Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody, Clone S48 (NBP2-12893). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody (NBP2-12893) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.![Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893]](https://resources.bio-techne.com/images/products/Cav1-3-Antibody-S48-Immunohistochemistry-NBP2-12893-img0001.jpg "Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893]")
Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893]
Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893] - IHC analysis of Cav1.3 tested on human hippocampus lysates. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.Applications for Cav1.3 Antibody (S48)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:1000
Western Blot
1:1000
Application Notes
1 ug/ml\L of Cav1.3 Antibody was sufficient for detection of Cav1.3 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cav1.3
Long Name
Voltage-dependent L-type calcium channel subunit alpha-1D
Alternate Names
CACH3, CACN4, CACNA1D, CACNL1A2, CCHL1A2
Gene Symbol
CACNA1D
Additional Cav1.3 Products
Product Documents for Cav1.3 Antibody (S48)
Product Specific Notices for Cav1.3 Antibody (S48)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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