Caveolin-2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99542

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Caveolin-2 Antibodies »

Product Summary for Caveolin-2 Antibody

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Caveolin-2

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Caveolin-2 Antibody

Western Blot: Caveolin-2 Antibody [NBP2-99542]

Western Blot: Caveolin-2 Antibody [NBP2-99542]

Western Blot: Caveolin-2 Antibody [NBP2-99542] - Anti-Caveolin-2 rabbit polyclonal antibody at 1:1000 dilution. Lane A: Mouse lung tissue lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 24 kDa (We are unsure as to the identity of these extra bands).x
Immunoprecipitation: Caveolin-2 Antibody [NBP2-99542]

Immunoprecipitation: Caveolin-2 Antibody [NBP2-99542]

Immunoprecipitation: Caveolin-2 Antibody [NBP2-99542] - Caveolin-2 was immunoprecipitated using: Lane A: 0.5 mg Mouse lung tissue Lysate 4 ul anti-Caveolin-2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-Caveolin-2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 22 kDa

Applications for Caveolin-2 Antibody

Application
Recommended Usage

Immunoprecipitation

5-10 uL/mg of lysate

Western Blot

1:1000-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caveolin-2

Caveolae are cholesterol/sphingolipid-rich microdomains of the plasma membrane that have been implicated in signal transduction and vesicular trafficking. Caveolins are a family of caveolae-associated integral membrane proteins (1). Caveolin, a 21- to 24-kDa integral membrane protein, is a principal component of caveolae membranes. Caveolin interacts directly with heterotrimeric guanine nucleotide binding proteins (G proteins) and can functionally regulate their activity. Caveolins 1 and 2 are similar in most respects, however, caveolin-1 and caveolin-2 differ in their functional interactions with heterotrimeric G proteins, possibly explaining why caveolin-1 and -2 are coexpressed within a single cell (2). Caveolin-2 is up-regulated in response to the mechanical injury of differentiated PC12 cells; up-regulation of caveolin-2 under these conditions is strictly dependent on continued treatment with NGF. Robust expression of caveolin-1 and -2 is also observed along the entire cell surface of DRG neurons, including high levels on growth cones. These findings demonstrate that neuronal cells express caveolins (1).

Alternate Names

CAV2, Caveolin2

Gene Symbol

CAV2

Additional Caveolin-2 Products

Product Documents for Caveolin-2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Caveolin-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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