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CBL Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28758

Novus Biologicals, part of Bio-Techne
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NBP1-28758

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Summary for CBL Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 675 and 725 of human Casitas B-lineage lymphoma proto-oncogene using the numbering given in entry NP_005179.2 (GeneID 867).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CBL Antibody

Western Blot: CBL Antibody [NBP1-28758]

Western Blot: CBL Antibody [NBP1-28758]

Western Blot: CBL Antibody [NBP1-28758] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-c-Cbl antibody NBP1-28758 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. c-Cbl was also immunoprecipitated by rabbit anti-c-Cbl antibody NBP1-28757, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

Applications for CBL Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CBL

The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation.

Long Name

Casitas B-lineage Lymphoma, E3 Ubiquitin Protein Ligase CBL

Alternate Names

CBL2, RNF55

Entrez Gene IDs

867 (Human)

Gene Symbol

CBL

Additional CBL Products

Product Documents for CBL Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CBL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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