CBL Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86588

Novus Biologicals, part of Bio-Techne
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NBP1-86588
NBP1-86588-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CBL Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PTTNVTEGSQVPERPPKPFPRRINSERKAGSCQQGSGPAASAATASPQLSSEIENLMSQGYSYQDIQKALVIAQNNIEMAKNILREFVSISSPAHVA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CBL Antibody

Immunohistochemistry-Paraffin: CBL Antibody [NBP1-86588]

Immunohistochemistry-Paraffin: CBL Antibody [NBP1-86588]

Immunohistochemistry-Paraffin: CBL Antibody [NBP1-86588] - Analysis in human testis and skeletal muscle tissues. Corresponding CBL RNA-seq data are presented for the same tissues.
Western Blot: CBL Antibody [NBP1-86588]

Western Blot: CBL Antibody [NBP1-86588]

Western Blot: CBL Antibody [NBP1-86588] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/ Immunofluorescence: CBL Antibody [NBP1-86588]

Immunocytochemistry/ Immunofluorescence: CBL Antibody [NBP1-86588]

Immunocytochemistry/Immunofluorescence: CBL Antibody [NBP1-86588] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
View 5 more images

Applications for CBL Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CBL

c-Cbl, product of c-cbl proto-oncogene, is a 120 kDa cytoplasmic protein involved in tyrosine kinase-dependent signaling pathways (1-3). c-Cbl is one of the earliest targets of tyrosine phosphorylation in response to a number of cellular stimuli. For instance, stimulation of growth factor receptors by EGF, PDGF, NGF and FGF, results in c-Cbl tyrosine phosphorylation, in lymphoid and other cell lines (4).

Long Name

Casitas B-lineage Lymphoma, E3 Ubiquitin Protein Ligase CBL

Alternate Names

CBL2, RNF55

Gene Symbol

CBL

Additional CBL Products

Product Documents for CBL Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CBL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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