CBP/KAT3A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-381
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, Western Blot
Cited:
Chemotaxis
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 975 and 1025 of human CREB Binding Protein using the numbering given in entry NP_004371.1 (GeneID 1387).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
265 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB100-1567, NB200-171, NB100-97831, NB100-58826
Scientific Data Images for CBP/KAT3A Antibody
Western Blot: CBP/KAT3A Antibody [NB100-381]
Western Blot: CBP/KAT3A Antibody [NB100-381] - Detection of Human CBP by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody NB100-381 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunoprecipitation: CBP/KAT3A Antibody [NB100-381]
Immunoprecipitation: CBP/KAT3A Antibody [NB100-381] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody used for IP at 6 ug/mg lysate. CBP was also immunoprecipitated by rabbit anti-CBP antibody NB100-382. For blotting immunoprecipitated CBP, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for CBP/KAT3A Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:10-1:500
Immunoprecipitation
1-4 ug/mg of lysate
Western Blot
1:1000 - 1:25000
Application Notes
This antibody immunoprecipitates CBP. It also recognizes a band at ~240 kDa in Western blot, representing CBP. It is strongly recommended that the investigator use nuclear extracts for optimal results. CBP/KAT3A antibody validated for Chip from a verified customer review and (PMID: 30137413).
Reviewed Applications
Read 2 reviews rated 4.5 using NB100-381 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CBP
Long Name
CREB-binding Protein
Alternate Names
CREBBP, RTS
Entrez Gene IDs
1387 (Human)
Gene Symbol
CREBBP
UniProt
Additional CBP Products
Product Documents for CBP/KAT3A Antibody
Product Specific Notices for CBP/KAT3A Antibody
This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB100-1567, NB200-171, NB100-97831, NB100-58826
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...