CBR1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1066

Novus Biologicals, part of Bio-Techne
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NB100-1066
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Porcine

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all CBR1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HGQFVSEKRVEQW corresponding to C-Terminus according to NP_001748.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CBR1 Antibody

Western Blot: CBR1 Antibody [NB100-1066]

Western Blot: CBR1 Antibody [NB100-1066]

Western Blot: CBR1 Antibody [NB100-1066] - Staining of Human Liver lysate (35 ug protein in RIPA buffer. Antibody at 0.03 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: CBR1 Antibody [NB100-1066]

Immunocytochemistry/ Immunofluorescence: CBR1 Antibody [NB100-1066]

Immunocytochemistry/Immunofluorescence: CBR1 Antibody [NB100-1066] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: CBR1 Antibody [NB100-1066]

Immunohistochemistry-Paraffin: CBR1 Antibody [NB100-1066]

Immunohistochemistry-Paraffin: CBR1 Antibody [NB100-1066] - Staining of paraffin embedded Human Kidney. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for CBR1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:4000

Western Blot

0.03 - 0.1 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CBR1

Carbonyl reductase is one of several monomeric, NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds. This enzyme is widely distributed in human tissues. Another carbonyl reductase gene, CRB3, lies close to this gene on chromosome 21q.

Alternate Names

15-hydroxyprostaglandin dehydrogenase [NADP+], carbonyl reductase (NADPH) 1, carbonyl reductase (NADPH) 1, EC 1.1.1.18410EC 1.1.1.197,15-hydroxyprostaglandin dehydrogenase, carbonyl reductase [NADPH] 1, carbonyl reductase 1, CBR, CRN, EC 1.1.1.184, EC 1.1.1.189, hCBR1, NADPH-dependent carbonyl reductase 1, Prostaglandin 9-ketoreductase, Prostaglandin-E(2) 9-reductase, SDR21C1, short chain dehydrogenase/reductase family 21C, member 1

Entrez Gene IDs

873 (Human)

Gene Symbol

CBR1

UniProt

NP_001748 (Human)

Additional CBR1 Products

Product Documents for CBR1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CBR1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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