CBS Antibody (1R2E9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15413
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1R2E9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for CBS Antibody (1R2E9)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 400-551 of human CBS (P35520). EDLTEKKPWWWHLRVQELGLSAPLTVLPTITCGHTIEILREKGFDQAPVVDEAGVILGMVTLGNMLSSLLAGKVQPSDQVGKVIYKQFKQIRLTDTLGRLSHILEMDHFALVVHEQIQYHSTGKSSQRQMVFGVVTAIDLLNFVAAQERDQK
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CBS Antibody (1R2E9)
Western Blot: CBS Antibody (1R2E9) [NBP3-15413]
Western Blot: CBS Antibody (1R2E9) [NBP3-15413] - Western blot analysis of extracts of various cell lines, using CBS Rabbit mAb (NBP3-15413) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Immunocytochemistry/ Immunofluorescence: CBS Antibody (1R2E9) [NBP3-15413]
Immunocytochemistry/Immunofluorescence: CBS Antibody (1R2E9) [NBP3-15413] - Immunofluorescence analysis of HeLa cells using CBS Rabbit mAb (NBP3-15413) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: CBS Antibody (1R2E9) [NBP3-15413]
Immunohistochemistry-Paraffin: CBS Antibody (1R2E9) [NBP3-15413] - Immunohistochemistry of paraffin-embedded human thyroid cancer using CBS Rabbit mAb (NBP3-15413) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for CBS Antibody (1R2E9)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CBS
Alternate Names
cystathionine-beta-synthase, EC 4.2.1.2210Beta-thionase
Gene Symbol
CBS
Additional CBS Products
Product Documents for CBS Antibody (1R2E9)
Product Specific Notices for CBS Antibody (1R2E9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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