CBX1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2423

Novus Biologicals, part of Bio-Techne
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NB100-2423
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all CBX1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QSQKTAHETDKSE corresponding to internal region according to NP_001120700.1.

Specificity

Variants (NP_006798.1; NP_001120700.1) encode the same protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CBX1 Antibody

Western Blot: CBX1 Antibody [NB100-2423]

Western Blot: CBX1 Antibody [NB100-2423]

Western Blot: CBX1 Antibody [NB100-2423] - (0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: CBX1 Antibody [NB100-2423]

Immunohistochemistry-Paraffin: CBX1 Antibody [NB100-2423]

Immunohistochemistry-Paraffin: CBX1 Antibody [NB100-2423] - 2.5ug/ml staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for CBX1 Antibody

Application
Recommended Usage

Immunohistochemistry

2 - 3 ug/ml

Immunohistochemistry-Paraffin

2 - 3 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 10 ug/ml
Application Notes
WB: Approx. 25 kDa band observed in lysates of A431, HeLa and HepG2 (calculated MW of 25 kDa band according to NP_006798.1). IHC-P: Human placenta shows strong nuclear staining in trophoblasts.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CBX1

HP1 gamma appears to be involved in transcriptional silencing in heterochromatin-like complexes. It recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. HP1 gamma may contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR) and involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Alternate Names

CBXM31HP1-BETA, chromobox homolog 1, chromobox homolog 1 (Drosophila HP1 beta), chromobox homolog 1 (HP1 beta homolog Drosophila ), chromobox protein homolog 1, Heterochromatin protein 1 homolog beta, heterochromatin protein 1-beta, Heterochromatin protein p25, heterochromatin protein p25 beta, HP1 beta, HP1 beta homolog, HP1Hsbeta, HP1Hs-beta, MOD1, Modifier 1 protein, p25beta

Entrez Gene IDs

10951 (Human); 12412 (Mouse); 360609 (Rat)

Gene Symbol

CBX1

UniProt

NP_006798.1 (Human)

Additional CBX1 Products

Product Documents for CBX1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CBX1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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