CBX1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98755

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CBX1 Antibodies »

Product Summary for CBX1 Antibody

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human CBX1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CBX1 Antibody

Western Blot: CBX1 Antibody [NBP2-98755]

Western Blot: CBX1 Antibody [NBP2-98755]

Western Blot: CBX1 Antibody [NBP2-98755] - Anti-CBX1 rabbit polyclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 23 kDa (We are unsure as to the identity of these extra bands).
Immunocytochemistry/ Immunofluorescence: CBX1 Antibody [NBP2-98755]

Immunocytochemistry/ Immunofluorescence: CBX1 Antibody [NBP2-98755]

Immunocytochemistry/Immunofluorescence: CBX1 Antibody [NBP2-98755] - Immunofluorescence staining of CBX1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CBX1 polyclonal antibody (dilution ratio 1:1000) at 4C overnight.
Immunoprecipitation: CBX1 Antibody [NBP2-98755]

Immunoprecipitation: CBX1 Antibody [NBP2-98755]

Immunoprecipitation: CBX1 Antibody [NBP2-98755] - CBX1 was immunoprecipitated using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate 2 ul anti-CBX1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-CBX1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa

Applications for CBX1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunoprecipitation

0.5-2 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CBX1

HP1 gamma appears to be involved in transcriptional silencing in heterochromatin-like complexes. It recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. HP1 gamma may contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR) and involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Alternate Names

CBXM31HP1-BETA, chromobox homolog 1, chromobox homolog 1 (Drosophila HP1 beta), chromobox homolog 1 (HP1 beta homolog Drosophila ), chromobox protein homolog 1, Heterochromatin protein 1 homolog beta, heterochromatin protein 1-beta, Heterochromatin protein p25, heterochromatin protein p25 beta, HP1 beta, HP1 beta homolog, HP1Hsbeta, HP1Hs-beta, MOD1, Modifier 1 protein, p25beta

Gene Symbol

CBX1

Additional CBX1 Products

Product Documents for CBX1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CBX1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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