CBX3 Antibody (1G12-1D9)
Novus Biologicals, part of Bio-Techne | Catalog # H00011335-M01
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1G12-1D9
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
CBX3 (AAH00954, 1 a.a. ~ 183 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASNKTTLQKMGKKQNGKSKKVEEAEPEEFVVEKVLDRRVVNGKVEYFLKWKGFTDADNTWEPEENLDCPELIEAFLNSQKAGKEKDGTKRKSLSDSESDDSKSKKKRDAADKPRGFARGLDPERIIGATDSSGELMLLMKWKDSDEADLVLAKEANMKCPQIVIAFYEERLTWHSCPEDEAQ
Reactivity Notes
Other species not tested.
Specificity
CBX3 - chromobox homolog 3 (HP1 gamma homolog, Drosophila)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for CBX3 Antibody (1G12-1D9)
![Western Blot: CBX3 Antibody (1G12-1D9) [H00011335-M01]](https://resources.bio-techne.com/images/products/CBX3-Antibody-1G12-1D9-Western-Blot-H00011335-M01-img0007.jpg "Western Blot: CBX3 Antibody (1G12-1D9) [H00011335-M01]")
Western Blot: CBX3 Antibody (1G12-1D9) [H00011335-M01]
Western Blot: CBX3 Antibody (1G12-1D9) [H00011335-M01] - CBX3 monoclonal antibody (M01), clone 1G12-1D9 Analysis of CBX3 expression in HeLa.![Immunocytochemistry/ Immunofluorescence: CBX3 Antibody (1G12-1D9) [H00011335-M01]](https://resources.bio-techne.com/images/products/CBX3-Antibody-1G12-1D9-Immunocytochemistry-Immunofluorescence-H00011335-M01-img0005.jpg "Immunocytochemistry/ Immunofluorescence: CBX3 Antibody (1G12-1D9) [H00011335-M01]")
Immunocytochemistry/ Immunofluorescence: CBX3 Antibody (1G12-1D9) [H00011335-M01]
Immunocytochemistry/Immunofluorescence: CBX3 Antibody (1G12-1D9) [H00011335-M01] - Analysis of monoclonal antibody to CBX3 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: CBX3 Antibody (1G12-1D9) [H00011335-M01] - Detection limit for recombinant GST tagged CBX3 is approximately 0.03ng/ml as a capture antibody.
Applications for CBX3 Antibody (1G12-1D9)
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: CBX3
Alternate Names
chromobox homolog 3, chromobox homolog 3 (Drosophila HP1 gamma), chromobox protein homolog 3, Drosophila), HECH, Heterochromatin protein 1 homolog gamma, heterochromatin protein HP1 gamma, heterochromatin-like protein 1, HP1 gamma, HP1 gamma homolog, HP1-GAMMA, Modifier 2 protein
Entrez Gene IDs
11335 (Human)
Gene Symbol
CBX3
OMIM
604477 (Human)
UniProt
Additional CBX3 Products
Product Documents for CBX3 Antibody (1G12-1D9)
Product Specific Notices for CBX3 Antibody (1G12-1D9)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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