CBX3 Antibody (7B7Q2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16368
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7B7Q2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for CBX3 Antibody (7B7Q2)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CBX3 (NP_057671.2). MASNKTTLQKMGKKQNGKSKKVEEAEPEEFVVEKVLDRRVVNGKVEYFLKWKGFTDADNTWEPEENLDCPELIEAFLNSQKAGKEKDGTKRKSLSDSESD
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CBX3 Antibody (7B7Q2)
![Western Blot: CBX3 Antibody (7B7Q2) [NBP3-16368]](https://resources.bio-techne.com/images/products/CBX3-Antibody-7B7Q2-Western-Blot-NBP3-16368-img0002.jpg "Western Blot: CBX3 Antibody (7B7Q2) [NBP3-16368]")
Western Blot: CBX3 Antibody (7B7Q2) [NBP3-16368]
Western Blot: CBX3 Antibody (7B7Q2) [NBP3-16368] - Western blot analysis of extracts of various cell lines, using CBX3 Rabbit mAb (NBP3-16368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.![Immunohistochemistry-Paraffin: CBX3 Antibody (7B7Q2) [NBP3-16368]](https://resources.bio-techne.com/images/products/CBX3-Antibody-7B7Q2-Immunohistochemistry-Paraffin-NBP3-16368-img0001.jpg "Immunohistochemistry-Paraffin: CBX3 Antibody (7B7Q2) [NBP3-16368]")
Immunohistochemistry-Paraffin: CBX3 Antibody (7B7Q2) [NBP3-16368]
Immunohistochemistry-Paraffin: CBX3 Antibody (7B7Q2) [NBP3-16368] - Immunohistochemistry of paraffin-embedded human colon using CBX3 Rabbit mAb (NBP3-16368) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.![Western Blot: CBX3 Antibody (7B7Q2) [NBP3-16368]](https://resources.bio-techne.com/images/products/CBX3-Antibody-7B7Q2-Western-Blot-NBP3-16368-img0003.jpg "Western Blot: CBX3 Antibody (7B7Q2) [NBP3-16368]")
Western Blot: CBX3 Antibody (7B7Q2) [NBP3-16368]
Western Blot: CBX3 Antibody (7B7Q2) [NBP3-16368] - Western blot analysis of extracts of Rat brain, using CBX3 Rabbit mAb (NBP3-16368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.Applications for CBX3 Antibody (7B7Q2)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CBX3
Alternate Names
chromobox homolog 3, chromobox homolog 3 (Drosophila HP1 gamma), chromobox protein homolog 3, Drosophila), HECH, Heterochromatin protein 1 homolog gamma, heterochromatin protein HP1 gamma, heterochromatin-like protein 1, HP1 gamma, HP1 gamma homolog, HP1-GAMMA, Modifier 2 protein
Gene Symbol
CBX3
Additional CBX3 Products
Product Documents for CBX3 Antibody (7B7Q2)
Product Specific Notices for CBX3 Antibody (7B7Q2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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