CBX3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83228

Novus Biologicals, part of Bio-Techne
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NBP1-83228
NBP1-83228-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CBX3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RVVNGKVEYFLKWKGFTDADNTWEPEENLDCPELIEAFLNSQKAGKEKDGTKRKSLSDSESDDSKSKKKRDAADKPRGFARGLDPERIIGATDSSGELMFLMKWKDSDEA

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:35417713). Reactivity reported in scientific literature (PMID: 23409045).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CBX3 Antibody

Immunohistochemistry-Paraffin: CBX3 Antibody [NBP1-83228]

Immunohistochemistry-Paraffin: CBX3 Antibody [NBP1-83228]

Immunohistochemistry-Paraffin: CBX3 Antibody [NBP1-83228] - Staining in human testis and pancreas tissues using anti-CBX3 antibody. Corresponding CBX3 RNA-seq data are presented for the same tissues.
Western Blot: CBX3 Antibody [NBP1-83228]

Western Blot: CBX3 Antibody [NBP1-83228]

Western Blot: CBX3 Antibody [NBP1-83228] - Analysis in human cell line SH-SY5Y.
Immunocytochemistry/ Immunofluorescence: CBX3 Antibody [NBP1-83228]

Immunocytochemistry/ Immunofluorescence: CBX3 Antibody [NBP1-83228]

Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NBP1-83228] - Staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.
View 2 more images

Applications for CBX3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CBX3

HP1 gamma appears to be involved in transcriptional silencing in heterochromatin-like complexes. It recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. HP1 gamma may contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR) and involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Alternate Names

chromobox homolog 3, chromobox homolog 3 (Drosophila HP1 gamma), chromobox protein homolog 3, Drosophila), HECH, Heterochromatin protein 1 homolog gamma, heterochromatin protein HP1 gamma, heterochromatin-like protein 1, HP1 gamma, HP1 gamma homolog, HP1-GAMMA, Modifier 2 protein

Gene Symbol

CBX3

Additional CBX3 Products

Product Documents for CBX3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CBX3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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