I learn from your company web that you are providing atf6, xbp1, cbx4 antibodies against human for immunofluorescence. And from the provided pictures, it seems very good. But I am wondering if anyone use them for immunofluorescence in human cells in your memory or in published papers? And how about them? By the way, you are providing free sample size for the atf6, xbp1 antibodies, aren't you? Could you send me some for tests? One more question, do they work on endogenous proteins but not only on overexpressed proteins?

These products have been validated for use in IF on human: catalog numbers NBP1-40256, NB100-80861, and NBP1-83225. Yes these antibodies should work on the native protein/endogenous protein.

We tested WB with NBP1-83225. The MW of this item was around 80kDa in the website. But we saw the result of 60kDa. When she searched paper and the other brand's datasheet, MW of CBX4 was 60kDa. We want to know the reason the difference from yours. Could you explain about it?

The predicted molecular weight of Human CBX4 protein is 61.6kDa. There are a number of reasons why predicted protein size does not always look that way on a Western blot. One has to consider post-translational modifications, splice variants, tissue specific processing, multimer formation and amino acid charge. To confirm specificity, a control such as recombinant protein or overexpression lysate, a downregulated knockdown/knockout lysate, a peptide competition of the primary antibody, or a treatment known to effect the expression of the protein should be used.

CBX4 Antibody

Name: CBX4 Antibody
Brand: Novus Biologicals
SKU: NBP1-83225

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83225

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Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CBX4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QPEVILLDSDLDEPIDLRCVKTRSEAGEPPSSLQVKPETPASAAVAVAAAAAPTTTAEKPPAEAQDEPAESLSEFKPFFGNIIITDVTANCLTVTFKEYVTV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (85%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CBX4 Antibody

Immunohistochemistry-Paraffin: CBX4 Antibody [NBP1-83225]

Immunohistochemistry-Paraffin: CBX4 Antibody [NBP1-83225] - Staining of human bone marrow shows strong nuclear positivity in subset of hematopoietic cells.

Applications for CBX4 Antibody

Application

Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CBX4

PC2 is the human homolog of the Drosophila 'Polycomb' (Pc) protein which has been identified as a gene family member associated with a cellular memory system that is responsible for the inheritance of gene activity by progeny cells. The human Pc homolog gene is more closely related to a Xenopus Pc homolog, XPc, than to a previously described human Pc homolog, CBX2 (hPc1). However, the hPc2 and CBX2/hPc1 proteins are shown to colocalize in interphase nuclei of human U-2 OS osteosarcoma cells, suggesting that the proteins are part of a common protein complex. The human protein is believed to function as a repressor of proto-oncogene activity and that interference with hPc2 function can lead to derepression of proto-oncogene transcription and subsequently to cellular transformation. Other reports describe PC2 as a protein that has SUMO E3 activity for the corepressors CtBP and CtBP2.

Alternate Names

chromobox homolog 4, chromobox homolog 4 (Drosophila Pc class), Chromobox protein homolog 4, Drosophila), E3 SUMO-protein ligase CBX4, hPc2, NS5ATP1-binding protein 16, Pc class 2 homolog, PC2, Polycomb 2 homolog

Gene Symbol

CBX4

Additional CBX4 Products

Product Documents for CBX4 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for CBX4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

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FAQs

CBX4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83225

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Concentration

View all CBX4 Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Clonality

Host

Isotype

Scientific Data Images for CBX4 Antibody

Immunohistochemistry-Paraffin: CBX4 Antibody [NBP1-83225]

Applications for CBX4 Antibody

Immunohistochemistry

Immunohistochemistry-Paraffin

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: CBX4

Alternate Names

Gene Symbol

Additional CBX4 Products

Product Documents for CBX4 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for CBX4 Antibody