Cbx8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-38870

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Chromatin Immunoprecipitation Sequencing, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Cbx8 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 350 and the C-terminus (residue 389) of human Chromobox homolog 8 using the numbering given in entry NP_065700.1 (GeneID 57332).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cbx8 Antibody

Western Blot: Cbx8 Antibody [NB110-38870]

Western Blot: Cbx8 Antibody [NB110-38870]

Western Blot: Cbx8 Antibody [NB110-38870] - Detection of Human CBX8 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CBX8 antibody NB110-38870 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Chromatin Immunoprecipitation Sequencing: Cbx8 Antibody [NB110-38870] - Localization of CBX8 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-CBX8 antibody NB110-38870 and analyzed by DNA sequencing. The figure illustrates the peak distribution of CBX8 binding within a 4 Mb region of chromosome 1 as detected using anti-CBX8 antibody NB110-38870. ChIP-seq validation performed by Diogenode, Denville, NJ.
Immunoprecipitation: Cbx8 Antibody [NB110-38870]

Immunoprecipitation: Cbx8 Antibody [NB110-38870]

Immunoprecipitation: Cbx8 Antibody [NB110-38870] - Detection of human CBX8 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CBX8 antibody NB110-38870 used for IP at 6 ug per reaction. CBX8 was also immunoprecipitated by rabbit anti-CBX8 antibody from Company B. For blotting immunoprecipitated CBX8, NB110-38870 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Cbx8 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cbx8

CBX8 is involved in maintaining the transcriptionally repressive state of genes. It modifies chromatin, rendering it heritably changed in its expressibility.

Alternate Names

chromobox homolog 8, chromobox homolog 8 (Drosophila Pc class), chromobox homolog 8 (Pc class homolog, Drosophila), HPC3, Pc3, PC3chromobox protein homolog 8, polycomb 3, Polycomb 3 homolog, RC1Pc class 3 homolog, Rectachrome 1

Entrez Gene IDs

57332 (Human)

Gene Symbol

CBX8

UniProt

Q9HC52 (Human)

Additional Cbx8 Products

Product Documents for Cbx8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cbx8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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