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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Coiled-coil and C2 Domain Containing 1A using the numbering given in TrEMBL entry Q6P1N0 (GeneID 54862).

Specificity

NB500-255 is specific for human TOUF/FLJ-20241 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CC2D1A Antibody

Western Blot: CC2D1A Antibody [NB500-255]

Western Blot: CC2D1A Antibody [NB500-255]

Western Blot: CC2D1A Antibody [NB500-255] - Detection of Human TOUF/FLJ20241 on HeLa whole cell lysate using NB500-255.
Immunohistochemistry-Paraffin: CC2D1A Antibody [NB500-255]

Immunohistochemistry-Paraffin: CC2D1A Antibody [NB500-255]

Immunohistochemistry-Paraffin: CC2D1A Antibody [NB500-255] - Human colon carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).
Western Blot: CC2D1A Antibody [NB500-255]

Western Blot: CC2D1A Antibody [NB500-255]

Western Blot: CC2D1A Antibody [NB500-255] - Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells

Applications for CC2D1A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

1 -4 ug/mg lysate

Western Blot

1:1000 - 1:10000
Application Notes
This antibody can be used in Western Blot, Immunofluorescence, and Immunoprecipitation. Recommended Starting Dilutions*: WB: 1:1,000-1:10,000 IP: 1-4 ug/mg lysate IF: 1/100 *Optimal dilutions should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CC2D1A

TOUF, or Cc2d1a, is a signal transducer that participates in the positive regulation of the I-kappaB kinase/NF-kappaB cascade. Cc2d1a has been implicated as playing an important role in human cognitive development, with a particular relation to Nonsyndromic Mental Retardation.

Alternate Names

Akt kinase-interacting protein 1, coiled-coil and C2 domain containing 1A, coiled-coil and C2 domain-containing protein 1A, Five repressor element under dual repression-binding protein 1, FLJ20241, FLJ41160, FRE under dual repression-binding protein 1, FREUD-1, mental retardation, nonsyndromic, autosomal recessive, 3, MRT3Freud-1/Aki1, Putative NF-kappa-B-activating protein 023N, putative NFkB activating protein

Entrez Gene IDs

54862 (Human)

Gene Symbol

CC2D1A

UniProt

Additional CC2D1A Products

Product Documents for CC2D1A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CC2D1A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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