CCDC124 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48858
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: LEDAYWKDDDKHVMRKEQRKEEKEKRRLDQLERKKETQRLLEEEDSKLKGGKAPRVATSSKVTRAQIEDTLRRDHQLRE
Reactivity Notes
Mouse (84%), Rat (82%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CCDC124 Antibody
Immunocytochemistry/ Immunofluorescence: CCDC124 Antibody [NBP2-48858]
Immunocytochemistry/Immunofluorescence: CCDC124 Antibody [NBP2-48858] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.Immunohistochemistry-Paraffin: CCDC124 Antibody [NBP2-48858]
Immunohistochemistry-Paraffin: CCDC124 Antibody [NBP2-48858] - Staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.Western Blot: CCDC124 Antibody [NBP2-48858] -
Western Blot: CCDC124 Antibody [NBP2-48858] - The expression of IL‐25 affects the cisplatin resistance of lung cancer cells. A, The expression of IL‐25 in A549 & A549/CDDP cells. B, IL‐25 overexpressed in A549 cells. C, silence of IL‐25 expression by siRNA in A549/CDDP cells. D, IL‐25 overexpression desensitizes A549 cells to cisplatin treatment. E, Silence of IL‐25 expression sensitizes A549/CDDP cells to cisplatin treatment. *P < 0.01 vs indicated controls, all figures represent the average of three sets of independent experiments Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31044552), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for CCDC124 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:2500 - 1:5000
Immunohistochemistry-Paraffin
1:2500 - 1:5000
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. Use in Western blot reported in scientific literature (PMID: 31044552).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CCDC124
Alternate Names
coiled-coil domain containing 124, coiled-coil domain-containing protein 124
Gene Symbol
CCDC124
Additional CCDC124 Products
Product Documents for CCDC124 Antibody
Product Specific Notices for CCDC124 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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