CCDC16 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97135

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CCDC16 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human CCDC16 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CCDC16 Antibody

Western Blot: CCDC16 Antibody [NBP2-97135]

Western Blot: CCDC16 Antibody [NBP2-97135]

Western Blot: CCDC16 Antibody [NBP2-97135] - Anti-CCDC16 rabbit polyclonal antibody at 1:500 dilution. Lane A: U251MG Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: NIH3T3 Whole Cell Lysate Lane D: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 50 kDa
Immunocytochemistry/ Immunofluorescence: CCDC16 Antibody [NBP2-97135]

Immunocytochemistry/ Immunofluorescence: CCDC16 Antibody [NBP2-97135]

Immunocytochemistry/Immunofluorescence: CCDC16 Antibody [NBP2-97135] - Immunofluorescence staining of CCDC16 in U251-MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CCDC16 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.
Immunohistochemistry-Paraffin: CCDC16 Antibody [NBP2-97135]

Immunohistochemistry-Paraffin: CCDC16 Antibody [NBP2-97135]

Immunohistochemistry-Paraffin: CCDC16 Antibody [NBP2-97135] - Immunochemical staining of human CCDC16 in human duodenum with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
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Applications for CCDC16 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

5-10 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CCDC16

CCDC16 (coiled-coil domain containing 16) is a U1-like C2H2-type zinc finger protein whose function has not been characterized. Coiled-coil proteins exhibit tertiary structures made up of a number of highly organized alpha helices wound around each other to mediate oligomerization.

Alternate Names

CCDC16, coiled-coil domain containing 16, Coiled-coil domain-containing protein 16, MGC20398, OMCG1, SEL13, sel-13 suppressor of lin-12 homolog, zinc finger protein 830

Gene Symbol

ZNF830

Additional CCDC16 Products

Product Documents for CCDC16 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CCDC16 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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