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CCL2/MCP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81837

Novus Biologicals, part of Bio-Techne
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NBP1-81837
NBP1-81837-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CCL2/MCP1 Antibody

Immunocytochemistry/ Immunofluorescence: CCL2/MCP1 Antibody [NBP1-81837]

Immunocytochemistry/ Immunofluorescence: CCL2/MCP1 Antibody [NBP1-81837]

Immunocytochemistry/Immunofluorescence: CCL2/MCP1 Antibody [NBP1-81837] - Staining of human cell line BJ shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CCL2/MCP1 Antibody [NBP1-81837]

Immunohistochemistry-Paraffin: CCL2/MCP1 Antibody [NBP1-81837]

Immunohistochemistry-Paraffin: CCL2/MCP1 Antibody [NBP1-81837] - Staining of human skin shows moderate positivity in plasma.
Immunohistochemistry-Paraffin: CCL2/MCP1 Antibody [NBP1-81837]

Immunohistochemistry-Paraffin: CCL2/MCP1 Antibody [NBP1-81837]

Immunohistochemistry-Paraffin: CCL2/MCP1 Antibody [NBP1-81837] - Staining of human skeletal muscle shows positivity in myocytes.

Applications for CCL2/MCP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CCL2/MCP1

Monocyte Chemoattracctant Protein-1 (MCP1), also known as CCL2, is produced by a wide range of cell types as a reaction to diverse inflammatory stimuli and is a chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. MCP1 augments monocyte anti-tumor activity and has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. In atherosclerosis MCP-1 has been implicated to be involved in the recruitment of monocytes into the arterial wall during the disease process.

Alternate Names

C-C motif chemokine 2, chemokine (C-C motif) ligand 2, GDCF-2, HC11Monocyte secretory protein JE, MCAFMonocyte chemotactic and activating factor, MCP1 Monocyte chemotactic protein 1, MCP-1 Small-inducible cytokine A2, MGC9434, Monocyte chemoattractant protein 1, monocyte chemoattractant protein-1, SCYA2HSMCR30, small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je), small inducible cytokine subfamily A (Cys-Cys), member 2, SMC-CF

Gene Symbol

CCL2

Additional CCL2/MCP1 Products

Product Documents for CCL2/MCP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CCL2/MCP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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