CCL3L1/LD78 beta Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-42775
Key Product Details
Species Reactivity
Porcine, Bovine
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This protein-A purified CCL3L1/LD78 beta Antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 70 amino acids of the mature swine CCL3L1/LD78 beta/LD78 beta protein. (Uniprot: A1YM34)
Reactivity Notes
A BLAST analysis was used to suggest cross-reactivity with CCL3L1/LD78 beta from bovine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against CCL3L1/LD78 beta from cat, dog, human, chimpanzee, baboon, bovine, and rat based on greater than 90% sequence homology. Cross-reactivity with CCL3L1/LD78 beta from other sources has not been determined.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This product was Protein-A purified from monospecific antiserum by chromatography
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for CCL3L1/LD78 beta Antibody
![Western Blot: CCL3L1/LD78 beta Antibody [NBP1-42775]](https://resources.bio-techne.com/images/products/LD78-beta-Antibody-Western-Blot-NBP1-42775-img0004.jpg "Western Blot: CCL3L1/LD78 beta Antibody [NBP1-42775]")
Western Blot: CCL3L1/LD78 beta Antibody [NBP1-42775]
Western Blot: LD78 beta Antibody [NBP1-42775] - Analysis of recombinant swine CCL3L1 at 7.8kDa (arrow) raised in yeast. Protein was purified and resolved by SDS-PAGE, transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with , Inc. Anti-swine CCL3L1. After washing, membrane was probed with Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody.
CCL3L1/LD78 beta Antibody
Western blot using protein-A purified anti-swine CCL3L1 antibody shows detection of recombinant swine CCL3L1 at 7.8kDa raised in yeast. Protein was purified and resolved by SDS-PAGE, transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with , Inc. Anti-swine CCL3L1. After washing, membrane was probed with Dylight (TM) 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody ().Applications for CCL3L1/LD78 beta Antibody
Application
Recommended Usage
ELISA
1:100-1:2000
Western Blot
1 ug/ml
Application Notes
This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 7.8 kDa in size corresponding to swine CCL3L1 protein by western blotting in the appropriate cell lysate or extract.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: CCL3L1/LD78 beta
CCL3L1/MIP-1 alpha acts as a chemoattractant to a variety of cells including monocytes, T cells, B cells and eosinophils. CCL3L1 binds to several chemokine receptors. In humans these receptors include chemokine binding protein 2 and chemokine (C-C motif) receptor 5 (CCR5). CCR5 is a co-receptor for HIV, and binding of this protein to CCR5 inhibits HIV entry. In swine, CCL3L1 protein is 86% similar to CCL4 protein, and 73% similar to CCL5.
Alternate Names
C-C motif chemokine 3-like 1, CCL3L3, chemokine (C-C motif) ligand 3-like 1, D17S1718, G0/G1 switch regulatory protein 19-2, G0S19-2LD78, LD78BETA, LD78-beta(1-70), MGC104178, MGC12815, MGC182017, MIP1AP, PAT 464.2, SCYA3L, SCYA3L1464.2, small inducible cytokine A3-like 1, Small-inducible cytokine A3-like 1, Tonsillar lymphocyte LD78 beta protein
Gene Symbol
CCL3L1
UniProt
Additional CCL3L1/LD78 beta Products
Product Documents for CCL3L1/LD78 beta Antibody
Product Specific Notices for CCL3L1/LD78 beta Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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