Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1E9

Concentration

0.38 mg/ml

View all CCM2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CCM2(NP_113631) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

48.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CCM2 Antibody (OTI1E9)

Western Blot: CCM2 Antibody (1E9) [NBP2-01134] Analysis of extracts (35ug) from 9 different cell lines by using anti-CCM2 monoclonal antibody.
Immunohistochemistry-Paraffin: CCM2 Antibody (OTI1E9) [NBP2-01134]

Immunohistochemistry-Paraffin: CCM2 Antibody (OTI1E9) [NBP2-01134]

Immunohistochemistry-Paraffin: CCM2 Antibody (1E9) [NBP2-01134] - Staining of paraffin-embedded Human lymph node tissue using anti-CCM2 mouse monoclonal antibody.
Western Blot: CCM2 Antibody (OTI1E9) [NBP2-01134]

Western Blot: CCM2 Antibody (OTI1E9) [NBP2-01134]

Western Blot: CCM2 Antibody (1E9) [NBP2-01134] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCM2.
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Applications for CCM2 Antibody (OTI1E9)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.38 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CCM2

This gene encodes a scaffold protein that functions in the stress-activated p38 Mitogen-activated protein kinase (MAPK) signaling cascade. The protein interacts with SMAD specific E3 ubiquitin protein ligase 1 (also known as SMURF1) via a phosphotyrosine binding domain to promote RhoA degradation. The protein is required for normal cytoskeletal structure, cell-cell interactions, and lumen formation in endothelial cells. Mutations in this gene result in cerebral cavernous malformations. Multiple transcript variants encoding different isoforms have been found for this gene.

Alternate Names

C7orf22OSM, cerebral cavernous malformation 2, Cerebral cavernous malformations 2 protein, chromosome 7 open reading frame 22, malcavernin, MGC4067, MGC4607, MGC74868

Entrez Gene IDs

83605 (Human)

Gene Symbol

CCM2

Additional CCM2 Products

Product Documents for CCM2 Antibody (OTI1E9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CCM2 Antibody (OTI1E9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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