CCR1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56334

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rabbit

Cited:

Human, Mouse, Rabbit

Applications

Validated:

Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow - IC, Flow (Cell Surface), Flow Cytometry, Flow Cytometry Control, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all CCR1 Antibodies »

Product Specifications

Immunogen

This antibody was raised against a synthetic peptide of human CCR1 protein.

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 20371596).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CCR1 Antibody

Western Blot: CCR1 Antibody [NB100-56334]

Western Blot: CCR1 Antibody [NB100-56334]

Western Blot: CCR1 Antibody [NB100-56334] - A375 lysate probed with this antibody at 2 ug/ml dilution.
Immunohistochemistry-Paraffin: CCR1 Antibody [NB100-56334]

Immunohistochemistry-Paraffin: CCR1 Antibody [NB100-56334]

Immunohistochemistry-Paraffin: CCR1 Antibody [NB100-56334] - Normal human spleen tissue stained with this at 5 ug/ml, peroxidase-conjugate and DAB chromogen. Note granulocyte staining.
Flow (Intracellular): CCR1 Antibody [NB100-56334]

Flow (Intracellular): CCR1 Antibody [NB100-56334]

Flow (Intracellular): CCR1 Antibody [NB100-56334] - An intracellular stain was performed on Raw264.7 cells with CCR1 Antibody NB100-56334 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).
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Applications for CCR1 Antibody

Application
Recommended Usage

Flow (Intracellular)

reported in scientific literature (PMID 20371596)

Flow Cytometry

5-10 ul/test

Immunohistochemistry-Frozen

reported in scientific literature (PMID 15860797)

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-3 ug/ml
Application Notes
In A375, a 42 kDa band should be observed.

Reviewed Applications

Read 1 review rated 4 using NB100-56334 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CCR1

Chemokines play important roles in inflammation and critical for the recruitment of effector immune cells to sites of infection. Chemokines activate leukocytes by binding to G protein coupled receptors (1). The ever-growing chemokine receptor subtypes can be divided into 2 major groups, CXCR and CCR, based on the 2 major classes of chemokines. One of the CCR receptors, CCR1, is expressed on neutrophils, monocytes, lymphocytes, and eosinophils and binds the leukocyte chemoattractant and hemopoiesis regulator macrophage-inflammatory protein (MIP-1), eotaxin, as well as several other related chemokines (2-4). Mice lacking the chemokine receptor CCR1 have defects in neutrophil trafficking and proliferation (5,6).

Alternate Names

CCR1, CD191

Entrez Gene IDs

1230 (Human); 12768 (Mouse)

Gene Symbol

CCR1

UniProt

NP_001286.1 (Human)

Additional CCR1 Products

Product Documents for CCR1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CCR1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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