CCR10/GPR2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56319

Novus Biologicals, part of Bio-Techne
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NB100-56319
NB100-56319SS
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow (Cell Surface), Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Simple Western, Western Blot

Cited:

Flow - IC, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all CCR10 Antibodies »

Product Specifications

Immunogen

Rabbit anti-CCR10 polyclonal antibody was raised against a peptide corresponding to amino acids 350-362 of human CCR10. In this region, mouse and human amino acid sequences are 100% identical.

Reactivity Notes

Predicted to react with Mouse.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CCR10/GPR2 Antibody

Western Blot: CCR10/GPR2 Antibody [NB100-56319]

Western Blot: CCR10/GPR2 Antibody [NB100-56319]

Western Blot: CCR10/GPR2 Antibody [NB100-56319] - Analysis for GPR2/CCR10 using NB100-56319 at 2 ug/ml dilution against 10 ug of MCF-7 (a human breast cancer cell line) cell lysate.
Simple Western: CCR10/GPR2 Antibody [NB100-56319]

Simple Western: CCR10/GPR2 Antibody [NB100-56319]

Simple Western: CCR10/GPR2 Antibody [NB100-56319] - Simple Western lane view shows a specific band for CCR10 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for CCR10/GPR2 Antibody

Application
Recommended Usage

Flow (Intracellular)

reported in scientific literature (PMID 15663561)

Flow Cytometry

1:10-1:1000

Immunohistochemistry-Frozen

1:10-1:2000. Use reported in scientific literature (Vestergaard et al (2003))

Simple Western

1:50

Western Blot

1-2 ug/ml
Application Notes
In A375, a ~50 kDa band is observed. The CCR10 antibody is made against an epitope in the cytoplasmic domain of the receptor (Moed et al, 2004). For flow cytometry (intracellular) Moed et al fixed cells with 2% formaldehye and permeabilized with 0.1% saponin.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS with 0.2% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CCR10

Recently, a function has been assigned to a previously identified orphan chemokine receptor, GPR-2. This receptor, also called CCR10, binds with monocyte chemoattractant protein (MCP)-1, MCP-3 and MIP-1 beta. CCR10 is expressed by melanocytes, dermal fibroblasts, dermal microvascular endothelial cells, T cells as well as in skin-derived Langerhans cells. CCR10 may play a role in both skin homeostasis as well as a potential role in inflammatory responses.

Alternate Names

CCR10, GPR2

Entrez Gene IDs

2826 (Human)

Gene Symbol

CCR10

UniProt

P46092

Additional CCR10 Products

Product Documents for CCR10/GPR2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CCR10/GPR2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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