CCR2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-48337

Novus Biologicals, part of Bio-Techne
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NBP1-48337
NBP1-48337SS
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Key Product Details

Species Reactivity

Validated:

Human, Rat, Bovine, Feline

Cited:

Human, Mouse, Rat, Bovine, Feline

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all CCR2 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an N-terminal portion of the human CCR2 protein (within residues 20-100). [Swiss-Prot# P41597]

Reactivity Notes

Feline reactivity reported in scientific literature (PMID: 30395163). Use in Bovine reported in scientific literature (PMID:31761882). Reactivity with Rat reported by verified customer. The immunogen sequence similarity with other species: Rhesus monkey and several other primate species (100%), Mouse (69%).

Localization

Multi-pass cell membrane protein with extracellular and cytoplasmic domains.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CCR2 Antibody - BSA Free

Immunohistochemistry: CCR2 Antibody - BSA Free [NBP1-48337]

Immunohistochemistry: CCR2 Antibody - BSA Free [NBP1-48337]

Immunohistochemistry: CCR2 Antibody [NBP1-48337] - Localization of CCR1 (binds to CCL8, CCL14, and CCL16), CCR2 (binds to CCL2, CCL8, and CCL16), CCR3 (binds to CCL11), and CXCR3 (binds to CXCL10) in the bovine endometrium and fetal trophoblast obtained from cows in their 18th day of pregnancy. Intensive immunoreactivity was observed in endometrial epithelial cells, glandular epithelial cells, or fetal trophoblast. No positive immunoreactivity was observed in the negative control (Control). Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/18/4/742), licensed under a CC-BY license.
Western Blot: CCR2 AntibodyBSA Free [NBP1-48337]

Western Blot: CCR2 AntibodyBSA Free [NBP1-48337]

Western Blot: CCR2 Antibody [NBP1-48337] - Analysis of CCR2 in rat lysate. Image courtesy of product review submitted by Rasha Elbaz.
Flow Cytometry: CCR2 Antibody - BSA Free [NBP1-48337]

Flow Cytometry: CCR2 Antibody - BSA Free [NBP1-48337]

Flow Cytometry: CCR2 Antibody - BSA Free [NBP1-48337] - An intracellular stain was performed on U2-OS cells with CCR2 NBP1-48337AF647 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
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Applications for CCR2 Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

5 ug/ml

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

2 ug/ml
Application Notes
This CCR2 antibody is useful for Flow Cytometry, Immunohistochemistry-Paraffin and Immunocytochemistry/Immunofluorescence applications. Some customers have also reported success using this antibody for Western Blot on rat and human lysates.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CCR2

CCR2 is a major chemokine receptor expressed on monocytes and its activation through its ligands MCP1, MCP2, MCP3 and MCP4 (systematic names CCL2, CCL8, CCL7 and CCL13, respectively) stimulates the migration of monocytes across the vascular wall into tissues wherein they facilitate chronic inflammation. CCR2 is a member of GPCR1 family and is up-regulated by CREB3. It is a multi-pass membrane protein found in cell membrane, and besides monocytes, it is also found on other cell types like NK cells, macrophages, immature dendritic cells, gamma delta T cells, and activated T cells (including Th17 cells). There are two alternatively spliced forms of CCR2: CCR2A - the major isoform expressed by mononuclear cells and vascular smooth muscle cells; and CCR2B - expressed predominantly by monocytes and activated NK cells. CCR2 has both pro-inflammatory (mediated by APC and T cells) and anti-inflammatory (mediated by regulatory T cells) activities. Besides inflammation, it has been implicated several pathologies including HIV-1/AIDS, autoimmune disorders (psoriasis, rheumatoid arthritis, and multiple sclerosis), pulmonary diseases (asthma, COPD), vascular disease and cancer (prostate cancer).

Alternate Names

CC-CKR-2, CCR2, CCR2B, CD192, CKR2, CKR2A, CMKBR2, FLJ78302, MCP-1-R

Entrez Gene IDs

729230 (Human)

Gene Symbol

CCR2

UniProt

P41597 (Human)

Additional CCR2 Products

Product Documents for CCR2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CCR2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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