Flow Cytometry: CCR2 Antibody [NBP1-48338] - An intracellular stain was performed on THP-1 cells with CCR2 Antibody NBP1-48338G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.

Immunocytochemistry/ Immunofluorescence: CCR2 Antibody - BSA Free [NBP1-48338]

Immunocytochemistry/Immunofluorescence: CCR2 Antibody [NBP1-48338] - Analysis of CCR2 in NIH/3T3 cells.

Immunohistochemistry: CCR2 Antibody - BSA Free [NBP1-48338]

Immunohistochemistry: CCR2 Antibody [NBP1-48338] - Expression patterns of CCL2 and CCR2 in unwounded and wounded corneas of WT and MMP12 KO mice. Immunofluorescence of CCL2 chemokine receptor CCR2 in unwounded and chemically wounded (2-days after injury) WT and Mmp12-/- mouse corneas. Control images represent mouse corneas stained with secondary antibody only and without primary antibody. Nuclei were visualized by staining with DAPI (blue). Scale bars: 50 um. CCR2 staining was visualized in epithelial and stromal layers of unwounded and wounded WT and Mmp12-/- corneas. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-019-47831-z), licensed under a CC-BY license.

Flow Cytometry: CCR2 Antibody - BSA Free [NBP1-48338]

Flow Cytometry: CCR2 Antibody [NBP1-48338] - An intracellular stain was performed on THP-1 cells with CCR2 Antibody NBP1-48338F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.

Flow Cytometry: CCR2 Antibody - BSA Free [NBP1-48338]

Flow Cytometry: CCR2 Antibody [NBP1-48338] - The Alexa Fluor 488 conjugate of this antibody was used: Mouse peripheral blood mononuclear cells were unstained (left) or stained (right) with CCR2 antibody. Image from verified customer review.

Detection of CCR2 in THP-1 Acute Monocytic Leukemia Human Cell Line by Flow Cytometry.

THP-1 human acute monocytic leukemia cell line was stained with Rabbit anti-CCR2 Affinity-purified Polyclonal Antibody conjugated to DyLight 650 (Catalog # NBP1-48338C, blue histogram) or matched control antibody (Catalog #NBP2-24891C, orange histogram).

CCR2 in NIH-3T3 Mouse Cell Line.

CCR2 was detected in immersion fixed NIH3T3 Mouse fibroblast cell line using Rabbit anti-CCR2 Antigen Affinity-purified Polyclonal Antibody conjugated to DyLight 550 (Catalog # NBP1-48338R) (red) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/ Immunofluorescence: CCR2 Antibody - BSA Free [NBP1-48338] -

Immunocytochemistry/ Immunofluorescence: CCR2 Antibody - BSA Free [NBP1-48338] - Expression patterns of CCL2 & CCR2 in unwounded & wounded corneas of WT & MMP12 KO mice. (A) Immunofluorescence of CCL2 chemokine & (B) its receptor CCR2 in unwounded & chemically wounded (2-days after injury) WT & Mmp12−/− mouse corneas. Control images represent mouse corneas stained with secondary antibody only & without primary antibody. Nuclei were visualized by staining with DAPI (blue). Scale bars: 50 µm. A magnified image of a wounded WT cornea shows perinuclear expression of CCL2 (orange box). CCL2 staining was visualized in epithelial, stromal, & endothelial layers of wounded WT & Mmp12−/− corneas. CCR2 staining was visualized in epithelial & stromal layers of unwounded & wounded WT & Mmp12−/− corneas. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31399604), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

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Applications for CCR2 Antibody - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:200

Immunohistochemistry-Frozen

reported in scientific literature (PMID 31399604)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CCR2

CCR2 is a major chemokine receptor expressed on monocytes and its activation through its ligands MCP1, MCP2, MCP3 and MCP4 (systematic names CCL2, CCL8, CCL7 and CCL13, respectively) stimulates the migration of monocytes across the vascular wall into tissues wherein they facilitate chronic inflammation. CCR2 is a member of GPCR1 family and is up-regulated by CREB3. It is a multi-pass membrane protein found in cell membrane, and besides monocytes, it is also found on other cell types like NK cells, macrophages, immature dendritic cells, gamma delta T cells, and activated T cells (including Th17 cells). There are two alternatively spliced forms of CCR2: CCR2A - the major isoform expressed by mononuclear cells and vascular smooth muscle cells; and CCR2B - expressed predominantly by monocytes and activated NK cells. CCR2 has both pro-inflammatory (mediated by APC and T cells) and anti-inflammatory (mediated by regulatory T cells) activities. Besides inflammation, it has been implicated several pathologies including HIV-1/AIDS, autoimmune disorders (psoriasis, rheumatoid arthritis, and multiple sclerosis), pulmonary diseases (asthma, COPD), vascular disease and cancer (prostate cancer).

Alternate Names

CC-CKR-2, CCR2, CCR2B, CD192, CKR2, CKR2A, CMKBR2, FLJ78302, MCP-1-R

Entrez Gene IDs

12772 (Mouse)

Gene Symbol

CCR2

UniProt

P51683 (Mouse)

Additional CCR2 Products

Product Documents for CCR2 Antibody - BSA Free

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download IHC-P Protocol

Download Protocol

COA

SDS

Download SDS

Product Specific Notices for CCR2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

CCR2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-48338

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all CCR2 Antibodies »

Product Specifications

Immunogen

Localization

Clonality

Host

Isotype

Scientific Data Images for CCR2 Antibody - BSA Free

Flow Cytometry: CCR2 Antibody - BSA Free [NBP1-48338]

Immunocytochemistry/ Immunofluorescence: CCR2 Antibody - BSA Free [NBP1-48338]

Immunohistochemistry: CCR2 Antibody - BSA Free [NBP1-48338]

Flow Cytometry: CCR2 Antibody - BSA Free [NBP1-48338]

Flow Cytometry: CCR2 Antibody - BSA Free [NBP1-48338]

Detection of CCR2 in THP-1 Acute Monocytic Leukemia Human Cell Line by Flow Cytometry.

CCR2 in NIH-3T3 Mouse Cell Line.

Immunocytochemistry/ Immunofluorescence: CCR2 Antibody - BSA Free [NBP1-48338] -

Applications for CCR2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Frozen

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: CCR2

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional CCR2 Products

Product Documents for CCR2 Antibody - BSA Free

Product Datasheets

Protocols & Manuals

COA

Certificate of Analysis

SDS

Product Specific Notices for CCR2 Antibody - BSA Free