CCR2 Antibody - BSA Free

Flow Cytometry: CCR2 Antibody - BSA Free [NBP1-48338]

Flow Cytometry: CCR2 Antibody [NBP1-48338] - An intracellular stain was performed on THP-1 cells with CCR2 Antibody NBP1-48338F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.

Flow Cytometry: CCR2 Antibody - BSA Free [NBP1-48338]

Flow Cytometry: CCR2 Antibody [NBP1-48338] - The Alexa Fluor 488 conjugate of this antibody was used: Mouse peripheral blood mononuclear cells were unstained (left) or stained (right) with CCR2 antibody. Image from verified customer review.

Detection of CCR2 in THP-1 Acute Monocytic Leukemia Human Cell Line by Flow Cytometry.

THP-1 human acute monocytic leukemia cell line was stained with Rabbit anti-CCR2 Affinity-purified Polyclonal Antibody conjugated to DyLight 650 (Catalog # NBP1-48338C, blue histogram) or matched control antibody (Catalog #NBP2-24891C, orange histogram).

CCR2 in NIH-3T3 Mouse Cell Line.

CCR2 was detected in immersion fixed NIH3T3 Mouse fibroblast cell line using Rabbit anti-CCR2 Antigen Affinity-purified Polyclonal Antibody conjugated to DyLight 550 (Catalog # NBP1-48338R) (red) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/ Immunofluorescence: CCR2 Antibody - BSA Free [NBP1-48338] -

Immunocytochemistry/ Immunofluorescence: CCR2 Antibody - BSA Free [NBP1-48338] - Expression patterns of CCL2 & CCR2 in unwounded & wounded corneas of WT & MMP12 KO mice. (A) Immunofluorescence of CCL2 chemokine & (B) its receptor CCR2 in unwounded & chemically wounded (2-days after injury) WT & Mmp12−/− mouse corneas. Control images represent mouse corneas stained with secondary antibody only & without primary antibody. Nuclei were visualized by staining with DAPI (blue). Scale bars: 50 µm. A magnified image of a wounded WT cornea shows perinuclear expression of CCL2 (orange box). CCL2 staining was visualized in epithelial, stromal, & endothelial layers of wounded WT & Mmp12−/− corneas. CCR2 staining was visualized in epithelial & stromal layers of unwounded & wounded WT & Mmp12−/− corneas. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31399604), licensed under a CC-BY license. Not internally tested by Novus Biologicals.