CCR5 Antibody (T21/8) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12086
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: CCR5 Antibody (T21/8) - Chimeric - Azide and BSA Free [NBP3-12086]](https://resources.bio-techne.com/images/products/CCR5-Antibody-T21-8-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12086-img0001.jpg "Immunocytochemistry/ Immunofluorescence: CCR5 Antibody (T21/8) - Chimeric - Azide and BSA Free [NBP3-12086]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # T21/8
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was raised by immunizing mice with intact RBL-CCR5 cells.
Specificity
T21/8 antibody binds with high affinity to a CCR5 N-terminal epitope. CCR5 is a receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for CCR5 Antibody (T21/8) - Chimeric - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: CCR5 Antibody (T21/8) - Chimeric - Azide and BSA Free [NBP3-12086]
Immunocytochemistry/Immunofluorescence: CCR5 Antibody (T21/8) - Chimeric [NBP3-12086] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version T21/8 (NBP3-12086) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12086, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.
Flow Cytometry: CCR5 Antibody (T21/8) - Chimeric - Azide and BSA Free [NBP3-12086] -
Flow Cytometry: CCR5 Antibody (T21/8) - Chimeric - Azide and BSA Free [NBP3-12086] - Paraformaldehyde fixed U937 cells were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12086 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.Applications for CCR5 Antibody (T21/8) - Chimeric - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: CCR5
CCR5 is a major coreceptor for human HIV infection and is predominantly expressed on T cells, macrophages, dendritic cells and microglia. It is likely that CCR5 plays a role in inflammatory responses to infection, though its exact role in normal immune function is unclear.
CCR5 antibodies are useful for HIV infection studies and research on immune responses.
Alternate Names
CCR5, CD195
Gene Symbol
CCR5
Additional CCR5 Products
Product Documents for CCR5 Antibody (T21/8) - Chimeric - Azide and BSA Free
Product Specific Notices for CCR5 Antibody (T21/8) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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