CD117/c-kit Antibody (007)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89411

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 007

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD117/c-kit Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD117/c-kit (Uniprot#: P10721-2; Met1-Thr516).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CD117/c-kit Antibody (007)

Immunocytochemistry/ Immunofluorescence: CD117/c-kit Antibody (007) [NBP2-89411]

Immunocytochemistry/ Immunofluorescence: CD117/c-kit Antibody (007) [NBP2-89411]

Immunocytochemistry/Immunofluorescence: CD117/c-kit Antibody (007) [NBP2-89411] - Staining of kit in HESS9 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human kit monoclonal antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).
Flow Cytometry: CD117/c-kit Antibody (007) [NBP2-89411]

Flow Cytometry: CD117/c-kit Antibody (007) [NBP2-89411]

Flow Cytometry: CD117/c-kit Antibody (007) [NBP2-89411] - Analysis of Human KIT(CD117) expression on TF-1 cells. Cells were stained with purified anti-Human KIT(CD117) (Filled hisgram), then a FITC-conjugated second step antibody. To demonstrate specificity of staining, the binding by was blocked by preincubation of the purified antibody with 20ug recombinant human KIT(CD117) for 1 hour (Black solid line hisgram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Applications for CD117/c-kit Antibody (007)

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD117/c-kit

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Gene Symbol

KIT

Additional CD117/c-kit Products

Product Documents for CD117/c-kit Antibody (007)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD117/c-kit Antibody (007)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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